Biosynthesis of CDP-choline by Immobilized Beer Yeast Cells
NIU Hong xing, QIU Wei ran *, DING Qing bao (State Key Laboratory of Bioreactor Engineering ECUST, Shanghai 200237, China)
Immobilized beer yeast cells entrapped in κ carrageenan konjak polysaccharide gel were used to synthesize CDP choline. In this experiment, 250mL Erlenmeyer flask with 50mL reaction liquid(400mmol/L glucose, 10mmol/L CMP, 50mmol/L choline phosphate, 100mmol/L K 2HPO 4 KH 2PO 4, 10mmol/L MgSO 4, pH=8.0) and 1 200g/L immobilized cells were used. After 4h incubation 400mmol/L of glucose was supplemented. The results showed that over 60% CMP was transformed to CDP choline after 8 hours incubation at 34°C and 150r/min. The reaction liquid being sterilized and NAD + added, the immobilized cells can be reused 4 times with yields over 40%.
【CateGory Index】： O629