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《Journal of East China University of Science and Technology(Natural Science Edition)》 2006-05
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Trend of Energy Charge and Oxidation-Reduction State in vivo during Pyruvate Production by Torulopsis glabrata 620

WANG Cui-hua,LI You-yuan,CHEN Chang-hua,LI Xiao(State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China)  
The method of determining ATP(adenosine-5'-triphosphate),ADP(adenosine-5'-dipphosphate),AMP(adenosine-5'-monophosphate),NAD~+(nicotinamide adenine dinucleotide) and NADH(nicotinamide adenine dinucleotide reduced) in vivo of pyruvate producing strain,Torulopsis glabrata 620 by high performance liquid chromatography(HPLC) was established and the profiles of both of energy charge and oxidationte-reduction state in vivo throughout the fermentation were studied.Energy charge in vivo plays a very important role in the biosynthesis of pyruvate.Pyruvate is accumulated intensively when the energy charge decreases to 0.1 and at the end of the production.It may represent a valuable tool to(improve) the production of pyruvate and to study the relationship between materials and energy in the cells.
【CateGory Index】: TQ920.1
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
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