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Determination of the Content of Puerarin in Yufeng Ningxin Tablet by HPLC

RUAN Hong-sheng,GOU Chun-qing,GAO Zhong-peng  
In order to determine the content of Puerarin in Yufeng Ningxin Tablet, HPLC was adopted. In this experiment ODS C18 column was used as column, methanol-water-glacial acetic acid (24.8:75:0.2) was used as the mobile phase, the detection wave-length was set at 250nm. The results showed there was a good linear relationship between consistence and apex-area when the detection range was 0.60~3.00ug. The regress equation was Y=5 840 436.333X-1 105 306.400, r=0.999 7. The recovery of the added sample were 99.82% and 101.23%, RSD were 0.98% and 1.04%.
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