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《Journal of Heilongjiang August First Land Reclamation University》 2007-04
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Optimization of Reaction System of RAPD for Magnaporthe grisea

LV Jun,JIN Xue-hui,HE Lin,YIN Kui-de,XEU Qing-zhi  
Using mycelium of Magnaporthe grisea as material, genomic DNA of Magnaporthe grisea was extracted by SDS-CTAB method. At the same time several factors which could affecte RAPD amplification in Magnaporthe grisea genomic DNA were analyzed and a better system was promoted. Results showed that the DNA distilled from mycelium had high quality. In 25μl reaction system, when annealing temperature was 34 ℃ , DNA template was 2.00μL, Taq was 1.25U, dNTPs was 1.60μL and concentration of material was1μL, electrophoresis bands were clear and stable.
【Fund】: 黑龙江省研究生创新科研项目(YJSCX2007-0252HLJ)。
【CateGory Index】: S435.111.41
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