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《Heilongjiang Agricultural Sciences》 2011-11
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Application of the Suppression Subtractive Hybridization Technique in Plant-Diseases

WANG Fang(Qiqihar Branch of Heilongjiang Academy of Agricultural Sciences,Qiqihar,Heilongjiang 161006)  
Based on the suppression PCR and subtractive hybridization,suppression subtractive hybridization(SSH)is a powerful technique that enables researchers to compare two populations of mRNA and obtain clones of genes that are expressed in one population but not in the other.SSH has become an efficient method that segregates and clone genes with the characteristics of high sensitivity,reliability and simplicity.The article introduced the principle and characteristics of SSH.The application on the study of plant-parasitic nematode and plant resistant-disease mechanism were reviewed.
【Fund】: 现代农业产业技术体系建设专项资金资助项目(CARS-004)
【CateGory Index】: S432
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【Citations】
Chinese Journal Full-text Database 9 Hits
1 ZHANG Shu-zhen1,XU Peng-fei1,WU Jun-jiang2,,LI Wen-bin1,CHEN Wei-yuan3,CHEN Chen1,YU An-liang1,WANG Jin-sheng1,JIN Li-mei1,LI Cen1(1Soybean Research Institute,Northeast Agricultural University;Chinese Education Ministry's Key Laboratory of Soybean Biology,Harbin 150030;2 Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences(HAAS),Harbin 150086;3 Suihua Research Institute of HAAS,Suihua 152052,Heilongjiang,China);Construction and Analysis of A SSH Library of Soybean Upon Infection with Phytophthora sojae[J];Soybean Science;2008-03
2 ZENG Yong-san,SUN Hui,LI Zhi-xuan,LIU Kai-qi(Plant Protection Department,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China);Construction of Chieh-qua Suppression Subtractive Hybridization cDNA Library Induced by Fusarium oxysporum f. sp. beniacasa[J];Hubei Agricultural Sciences;2010-03
3 ZHANG Chun-ying,WANG Xing-fen,ZHANG Gui-yin,WU Li-qiang,CHI Ji-na,LI Zhi-kun,MA Zhi-ying (Key Laboratory of Crop Germplasm Resources of Hebei/ Agricultural University of Hebei,Baoding,Hebei 071001,China);ESTs Analysis of Suppression Subtractive Hybridization Library from Upland Cotton Resistant Cultivar Infected by Verticillium dahliae[J];Cotton Science;2010-01
4 HUANG Xue-ling1, YU Xiu-dao1, QU Zhi-peng1, WANG Xiao-jie1, HAN Qing-mei2, HUANG Li-li1, KANG Zhen-sheng1,2 (1. College of Plant Protection,Northwest Agriculture and Forestry University, Yangling 712100,China; 2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling,712100,China);Construction of Suppression Subtractive Hybridization cDNA Library of Wheat Adult Plant Resistance to Stripe Rust and Analysis of Its Expressed Sequence Tags[J];Journal of Agricultural Biotechnology;2007-06
5 TIAN Zhen Dong 1,LIU Jun 2,XIE Cong Hua 1,① (1 College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China; 2 College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China);Isolation of Resistance Related-genes to Phytophthora infestans with Suppression Subtractive Hybridization in the R-gene-free Potato[J];Acta Genetica Sinica;2003-07
6 MAO Zhen-chuan1,2,XIE Bing-yan2,YANG Yu-hong2,FENG Dong-xin2,FENG Lan-xiang2,and YANG Zhi-wei1(1 College of Plant Protection,Northwest A & F University,Yangling,Shaanxi 712100,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Analysis of Early Expression Genes Resistance to Root Knot Nematode in N Gene Pepper by SSH[J];Acta Horticulturae Sinica;2007-03
7 ZHU Yan,WANG Chao (College of Horticulture,Northeast Agricultural University,Harbin 150030,Heilongjiang,China);Studies on Isolation of Resistance Related-genes to Black Rot by Suppression Subtractive Hybridization in Brassica oleracea[J];China Vegetables;2010-10
8 YU Xiu-mei2,YU Xiu-dao1,QU Zhi-peng1,HAN Qing-mei3,GUO Jun1,HUANG Li-li1,KANG Zhen-sheng1,3(1College of Plant Protection,Northwest A&F University,Yang-ling 712100,China;2College of Life Science,Northwest!A&F University,Yangling 712100,China;3Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A&F University,Yangling 712100,China);Construction of wheat SSH cDNA library induced by Puccinia striiformis and analysis of expressed sequence tags[J];Acta Phytopathologica Sinica;2007-01
9 TANG Yong-Qia1,2,YU Shuan-Cang2,ZHU Yue-Lin1,ZHANG Feng-Lan2,YU Yang-Jun2,ZHAO Xiu-Yun2,ZHANG De-Shuang2 1College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China;2Biejing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;Construction and Analysis of Suppression Subtractive Hybridization cDNA Library in Chinese Cabbage(Brassica rapa ssp.pekinensis) Leaves Induced by Peronospora parasitica[J];Plant Physiology Communications;2010-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SUN Hui et al(Department of Plant Protection,Zhongkai University of Agriculture and Engineering,Guangdong,Guangzhou 510225);Application of Subtractive cDNA Library in Study of Plant Resistance[J];Journal of Anhui Agricultural Sciences;2010-05
2 Cai Yingfan; Mo Jianchuan; Zeng Yu; Ren Weiwei; Miao Chen; Xu Ying;Wang Shenghua; Chen Fang.Life College of Sichuan University, Chengdu,610064,P.R.China.;Cloning of cDNAs associated with the development of pigment gland of Gossypium by suppression subtractive hybridization.[J];Journal of Beijing Forestry University;2003-03
3 XIAO Zhongjiu1,LI Xiaoxia2,ZHANG Suqin1,JIANG Xuanli1(1.Academy of Agriculture,Guizhou University,Guizhou 550025;2.Department of Biology,Zunyi Normal College);Application of SSH to Disease-resistance Gene Expression of Pepper[J];Journal of Changjiang Vegetables;2009-12
4 CHEN Rugang, GONG Zhenhui, LU Minghui, LI Dawei, HUANG Wei ( College of Horticulture, Northwest Agriculture and Forest University, Yangling, Shanxi, 712100 );Development and Prospect of the Gene Cloning Technology in Plant[J];Journal of Changjiang Vegetables;2009-20
5 CHEN Zhao-Yu1 XU An-Ying2 LI Mu-Wang2 ZHAO Yun-Po3 ZHOU Bo3SUN Ping-Jiang2 QIAN He-Ying2 ZHANG Yue-Hua2 LI Gang1 GUO Xi-Jie1,2(1College of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang Jiangsu 212018,China;2Sericultural Research Institute,Chinese Academy of Agricultural Sciences,Zhenjiang Jiangsu 212018,China;3Institute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China);SSH cDNA Library Construction and Corresponding EST Analysis of NILs for Yellow Hemolymph Gene in Silkworm,Bombyx mori[J];Science of Sericulture;2009-01
6 LI Liang,HOU Wen-sheng(National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI),Institute of Crop Sciences,The Chinese Academy of Agricultural Sciences,Beijing 100081,China);Application of Suppression Subtractive Hybridization in Soybean Research[J];Soybean Science;2010-04
7 ZHANG Hong,HU Yin-gang,REN Zhi-long,WANG Chang-you,WANG Ya-juan,JI Wan-quan*(College of Agronomy,Northwest A & F University,Yangling Branch of China Wheat Improvement Center,Shaanxi Key Lab of Molecular Biology for Agriculture,Yangling,Shaanxi 712100,China);Study on Gene Expression Inhibited by Innoculation of Puccinia striiformis West. in 'Shaanmai 139'[J];Acta Botanica Boreali-Occidentalia Sinica;2008-02
8 XIE Wei-wei~(1),WANG Ping-qing~(1),YANG Qing-chuan~(2),LIU Bo~(1),LI Zhi-zhong~(1)(1.College of Bioengineering,Chongqing University,Chongqing 400030,China;2.Institute of Animal Sciences of Chinese Academy of Agricultural Sciences,Beijing 100094,China);Methods for Plant Differential Expressed Gene Cloning and Progress[J];Journal of Chongqing University(Natural Science Edition);2005-12
9 Wang Junjun Wang Yingying Pan Haitao Li Sishen National Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Tai'an,271018;Construction and Analysis of SSH cDNA Library for Large Grain Mutant in Wheat[J];Molecular Plant Breeding;2010-01
10 ZHU Xiao-qin,SHEN Yuan-yue,FENG Yong-qing,QIN Ling(College of Plant Science and Technology,Beijing Agricultural College,Beijing 102206 China);Isolation of sport-related genes via suppression subtractive hybridization in short-catkin chestnut[J];Journal of Fruit Science;2009-03
China Proceedings of conference Full-text Database 1 Hits
1 FAN Shuguo Department of Chemistry and Life Science, Chuxiong Normal University, Chuxiong Yunna 675000, China;Advances in Epidemiology and Disease Management Measures of Potato Late Blight[A];[C];2008
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 JIN Li-mei1,XU Peng-fei1,WU Jun-jiang2,LI Wen-bin1,QIU Li-juan3,CHANG Ru-zhen3,CHEN Wei-yuan4,YU An-liang1,WANG Jin-sheng1,NAN Hai-yang1,CHEN Chen1 ,HANG Ying-peng1,CHEN Yan-qiu5,DING Guang-zhou1,ZHANG Shu-zhen1*(1.Soybean Research Institute,Northeast Agricultural University,Harbin Heilongjiang 150030;2.Soybean Institute,Heilongjiang Province of Agriaultaral Science,Harbin 150086;3.Crop Science Institute,Chinese Academy of Agricultural Sciences,Beijing,100081;4.Suihua Research Institute of Agriculture Sciences Academy of Heilongjiang Province,Suihua 152052;5.Crop Research Institute,Academy of Agricultural Sciences of Liaoning Province,Shenyang 110161);IDENTIFICATION THE RESISTANCE OF WILD SOYBEAN GERMPLASM TO PHYTOPHTHORN SOJAE[J];Soybean Science;2007-03
2 ZHANG Shu-zhen1,XU Peng-fei1,WU Jun-jiang2,,LI Wen-bin1,CHEN Wei-yuan3,CHEN Chen1,YU An-liang1,WANG Jin-sheng1,JIN Li-mei1,LI Cen1(1Soybean Research Institute,Northeast Agricultural University;Chinese Education Ministry's Key Laboratory of Soybean Biology,Harbin 150030;2 Soybean Research Institute,Heilongjiang Academy of Agricultural Sciences(HAAS),Harbin 150086;3 Suihua Research Institute of HAAS,Suihua 152052,Heilongjiang,China);Construction and Analysis of A SSH Library of Soybean Upon Infection with Phytophthora sojae[J];Soybean Science;2008-03
3 Xu XiuhongLu HuiyingYang QingkaiZhang XipingShang ChengweiZhang GuizhengFu Liheng (Soybean Research Institute, Northeast Agricultural University, Harbin, 150030);SCREENING OF SOYBEAN GERMPLASMS FORRESISTANCE TO PHYTOPHTHORA SOJAE[J];SOYBEAN SCIENCE;1999-02
4 Ma ShumeiLi BaoyingX(Heijiang Agricultural Institute, Heilongjiang Academy ofAgricullural Sciences, Jiamushi, 154007);A PRELMINARY REPORT ON THE IDENTIFICATION OFTHE PHYSIOLOGICAL RACES OF PHYTOPHTHORA MEGASPERMA[J];SOYBEAN SCIENCE;1999-02
5 LIU Tian-ming,HU Yin-gang,ZHANG Hong,SONG Guo-qi,LIN Fan-yun,JI Wan-quan~*(College of Agronomy,Northwest Sci-Tech University of Agriculture and Forestry,Yangling Branch of China Wheat Improvement Center;Shaanxi Key Laboratory of Molecular Biology for Agriculture,Yangling,Shaanxi 712100,China);Puccinia striiformis West-Induced Gene Expression of Wheat Germplasm with Stripe Rust Resistance[J];Acta Botanica Boreali-Occidentalia Sinica;2006-03
6 ZHANG Hong,HU Yin-gang,REN Zhi-long,WANG Chang-you,WANG Ya-juan,JI Wan-quan*(College of Agronomy,Northwest A & F University,Yangling Branch of China Wheat Improvement Center,Shaanxi Key Lab of Molecular Biology for Agriculture,Yangling,Shaanxi 712100,China);Study on Gene Expression Inhibited by Innoculation of Puccinia striiformis West. in 'Shaanmai 139'[J];Acta Botanica Boreali-Occidentalia Sinica;2008-02
7 LIN Fan-yun,LU Qiong-xian,XU Jian-hong,YANG Hui-yong,JI Fang,SHI Jian-rong(Institute of Food Safety Research and Inspection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China);Isolation of Genes Involved in the Response of Wheat to Fusarium graminearum by Means of Suppression Subtractive Hybridization[J];Acta Botanica Boreali-Occidentalia Sinica;2008-03
8 ZHANG Zhi-gang1,2,3,QIU De-wen2*,YANG Xiu-fen2,Zeng Hong-mei2,YUAN Jing-jing2,LIU Zheng2,YANG Xiao-ping3,GUAN Chun-yun2(1.Hunan Agriculture University,Changsha 410128,China;2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Hunan Cotton Research Institute,Changde 415000,China);Construction of Suppression Subtractive Hybridization Library with cDNA from Seedling Cotton Induced by Protein Elicitor from Alternaria tenuissima and Analysis of EST[J];Cotton Science;2007-04
9 YANG Yu-wen, ZHANG Bao-long, NI Wan-chao, SHEN Xin-lian, ZHANG Xiang-gui, XU Ying-jun(Institute of Agriculture Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China);Molecular Cloning and Expression Analysis of Two Chitinase in Upland Cotton[J];Cotton Science;2008-02
10 HUANG Xue-ling1, YU Xiu-dao1, QU Zhi-peng1, WANG Xiao-jie1, HAN Qing-mei2, HUANG Li-li1, KANG Zhen-sheng1,2 (1. College of Plant Protection,Northwest Agriculture and Forestry University, Yangling 712100,China; 2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling,712100,China);Construction of Suppression Subtractive Hybridization cDNA Library of Wheat Adult Plant Resistance to Stripe Rust and Analysis of Its Expressed Sequence Tags[J];Journal of Agricultural Biotechnology;2007-06
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