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《Heilongjiang Animal Science and Veterinary Medicine》 2012-07
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Isolation and culture of bovine mammary epithelial cells

LI Ji-xia1,GE Xiu-guo1,WANG Wen-xiu2,ZHANG Yong3(1.Department of Animal Science,Tianjin Agricultural University,Tianjin 300384,China;2.Shandong Binzhou Animal Science& Veterinary Medicine Institute,Binzhou 256600,China;3.College of Animal Veterinary Science,Northwest A&F University,Yangling 712100,China)  
To isolate,culture and identificatify the bovine mammary epithelial cells(MECs),studying the secrete function of the cells.The bovine mammary epithelial cells isolated with collagenase Ⅲ were purified using the passage methods.The cell marker proteins were determined with immunofluorescence staining,and the secrete function of MECs was identified by RT-PCR and in vitro induction.The results showed that the isolated bovine MECs had the typical morphological features,expressed a broad-spectrum of keratins,and secreted β-casein after induction.
【Fund】: 国家高技术研究发展计划项目(2008AA101006);; 天津市高等学校科技发展基金计划项目(20100619)
【CateGory Index】: S823
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