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《Hunan Forestry Science & Technology》 2016-05
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Diversity of nitrogen-fixing bacteria isolated from Pinus tabulaeformis in Helan Mountains

NIU Yanfang;CHEN Lihong;YAN Wei;Inner Mongolia Agricultural University;  
The diversity and community structure of nitrogen-fixing bacteria isolated from Pinus tabulaeformis rhizosphere in Helan Mountains was studied. Nitrogen-fixing bacteria from rhizosphere soil were isolated and purified by Ashby' s Medium,the 16 S r DNA gene of nitrogen-fixing bacteria was amplified using primers 27 f /1492 r and sequenced,the alignment of 16 S r DNA sequences was conducted by DNAMAN 6. 0,and the neighbor-joining phylogenetic tree was constructed by MEGA 4. 0 software. The results showed that nitrogen-fixing bacteria isolated from P. tabulaeformis belonged to nine groups,which were Pseudomonas, Bacillus, Phyllobacterium, Arthrobacter, Rhizobium, Paenibacillus, Sphingomonas,Caulobacter,Pedobacter,and Pseudomonas,Bacillus,Phyllobacterium,Arthrobacter were dominant groups.The results indicated that nitrogen-fixing bacteria from P. tabulaeformis in Helan Mountains have rich diversity.
【Fund】: 国家自然科学基金项目(31260173)
【CateGory Index】: S714.3
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