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《Journal of Basic and Clinical Oncology》 2012-04
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The Effect of Silencing Snail by siRNA on Generation and Invasion Capability of Gastric Cancer SGC7901 Cell Line

Ren Yanzhai1,Wang Feng2(1.Department of Surgery,the Third People's Hospital of Sanmenxia,Sanmenxia 472143,China; 2.Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)  
Objective To explore the effect of silencing Snail by siRNA on the generation and invasion capability of cell line SGC7901 in vitro,and to supply a specific target in gene therapy of gastric cancer.Methods Synthesized siRNA targeting Snail were transfected into SGC7901 cells,at the same time,SGC7901 cells transfected with negative siRNA,SGC7901 cells transfected with Lipofectamine 2000 and vacant SGC7901 cells were used as controls.RT-PCR was used to observe the transfection efficiency.MTT was used to evaluate the generation capability and Boyden chamber was used to evaluate the invasion capability of SGC7901 cells in vitro.Results Compared to all the control groups,SGC7901 cells transfected with Snail siRNA can suppress the expression of Snail mRNA in vitro,and along with the interfering time extending,Snail mRNA decreased gradually(P0.05).The results of MTT showed that the generation ability of SGC7901 cells decreased greatly after transfected Snail siRNA for 48 and 72 h compared to those in all the control groups(P0.05);the results of Boyden chamber also showed that the invasion ability of SGC7901 cells decreased greatly after transfected Snail siRNA for 48 and 72 h compared to those in all the control groups(P0.05);and there were significant variance among 24,48 and 72 h(P0.05).Conclusion Snail play an important role in the generation,development and invasion of gastric cancer,and may be a potentially valuable therapeutic target of gastric cancer.
【CateGory Index】: R735.2
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