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《Henan Science》 2006-05
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The Study on the Technique of Embryo Culture of Grapes(Ⅰ)

LIU Hai-lin1,2,LIU Zhan-jun1,WU Guo-xin1,LIAO Kang3,CHEN Li-geng2(1.College of Forestry Vocation,Henan Sci-Tech University,Luoyang 471002,China;2.Department of Horticulture,Zhejiang University,Key Laboratory of Horticultural Plant Growth,Development and Biotechnology,Ministry Agriculture,Hangzhou 310029,China;3.College of Horticulture,Xinjiang Agricultural University,Urumqi 830052,China)  
Young embryos of Thompson Seedless(v.vinifera cv.Sultanina) grapes of the 50 days after florescence,and Kyoho(V.vinifera×V.labrusce cv.),Rizamat(V.vinifera cv.) grapes of the 60 days after florescence were cultured on MS medium supplemented with different concentrations of GA3,IAA and 6-BA in order to study the techniques of embryo culture.The results indicated that the best media of inoculation of embryos of Thompson Seedless,Kyoho and Rizamat grapes were selected.We got 44±8.9%,96.0±8.9% development rate and 31.9±6.4%,37.5% germinating rate(p0.01) of Thompson Seedless' and Kyoho's embryo in the medium with MS+GA3 0.5mg·L-1 + IAA 2.0mg·L-1 + 6-BA 0.5mg·L-1,76.0±8.9% development rate and 63.5 ± 5.4% germinating rate(p 0.01) of Rizamat's embryos in the medium with MS + GA3 0.5mg·L-1+ IAA 0.5mg·L-1 + 6-BA 0.5mg·L-1.
【Fund】: 国家自然科学基金资助项目(30070634)
【CateGory Index】: S663.1
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