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《Journal of South China University of Technology(Natural Science Edition)》 2006-03
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Separation,Purification and Characteristics of Xylanase Separated from Trichoderma Koningii

Lu Deng-jun Xiao Kai-jun Guo Si-yuan Zheng Bi-sheng Cai Miao-yan (Research Institute of Light Industry and Chemical Engineering, South China Univ.of Tech.,Guangzhou 510640,Guangdong,China)  
Xylanase was separated and purified from a culture filtrate of Trichoderma Koningii through ammonium sulfate precipitation,Sephadex G-25 and Sephadex G-100 column chromatography.The purified xylanase was then proved by SDS-PAGE to be a single composition with a relative molecular mass of 55208.Good thermostability of xylanase with an optimal activity at 65℃ and pH 6.0 was also revealed.The results show that more than 83% or 85% of the relative activity of xylanase can be remained when respectively stored at 30~60℃ for 2h or in the pH range of 3.0~10.0.Moreover,Ba~(2+),Pb~(2+),Fe~(2+),Fe~(3+),Al~(3+) and 12mmol/L Cu~(2+) are strong inhibitors to xylanase,while Ca~(2+),Zn~(2+) and 4mmol/L Cu~(2+) are stimulators.The Michaelis-Menten constant of xylanases in the enzymatic hydrolysis of birchwood xylan is 5.37g/L,and the maximal reaction velocity is 0.94μmol/min.
【Fund】: 广东省科技攻关项目(2004B10201001);; 广东省科技计划项目(2004B20401001 2003B31701);; 广州市科技攻关项目(2002Z3-E0321)
【CateGory Index】: Q814.1
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