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《Journal of Henan Forestry Science and Technology》 2009-01
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Large flat apricotTissue CultureBasic medium And Culture conditions of Optimization

ZHANG Xiao-hang(Luoyang City Forestry Bureau Zhoushan Forest Park Management Office,Henan 471000,China)  
Large flat apricot Longwangmao were used to study the explant disinfection,basic medium,based on location and culture conditions and other factors on growth of plantlets.The results show that the 1 g/L HgCl2and20 g/L NaHCO3 Mixed by volume ratio of 1:1 mixture of disinfection treatment Explant8 min,Sterilization success rate of up to 98%;to modified MS(1/2NH4NO3) medium as the basic medium,Suitable for large flat apricot cultivate differentiation;the most suitable for shoot tip explants,the best culture conditions for the temperature 25~27 ℃,light intensity 2 000~3 000 lx,illumination time 16 h / d,PH 5.6~5.8.
【CateGory Index】: S662.2
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