Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of South China Agricultural University》 2001-01
Add to Favorite Get Latest Update

Primary Studies on Inheritance and Expression of Foreign Gene in Transgenic Rice

JIAN Yuyu1, CHEN Yuanling1, LI Jing1, GAO Lili1, DONG Chun2 (1 College of Biotechnology, South China Agric.Univ.,Guangzhou 510642, China; 2 College of Resources & Environmental Sciences, South China Agric. Univ., Guangzhou 510642  
Two transgenic plants transformed with cecropin B gene using biolistic method were generated from germinative embryo of rice variety TN 1. The inheritance and expression of bar and cecropin B gene in different transgenic plantlines of TN 1 from 5 generation of T 1 to T 5 were primary studied. Results showed that the segregation ratio of barR:barS was 3∶1 . The results of PCR and Southern blot analysis indicated that eight of T 1 and four of T 4 transgenic lines had cecropin B gene, the other plantlines of T 4 showed lost of cecropin gene. One of T 3 transgenic line expressed at mRNA level by Northern blot. The resistance to bacterial blight delivered from cecropin B transgene could be segregated and transmitted to high generation, resistance improved plantlines were obtained in T 3 generation.
【Fund】: 博士点基金!资助项目 (940 5 0 5 );; 华南生物科学与技术研究中心!资助
【CateGory Index】: S511
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 10 Hits
1 Mo Changming He Longfei* Liu Ming Li Chuangzhen Lu Sheng'an Wang Aiqin Li ZhigangCollege of Agronomy, Guangxi University, Nanning, 530005;Study on Agronomic Traits and Breeding Utilization of Transgenic Rice[J];Molecular Plant Breeding;2005-04
2 Lin Chunjing1,2 Dong Yingshan1* Lin Xiufeng1 Wang Jichun3 Tian Wenzhong4 Chu Chengcai41 Center of Agri-Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 2 Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024; 3 Institute of Plant Protect, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 4 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101;Primary Studies on Inheritance and Expression of Resistance to Rice Blast in Transgenic Rice[J];Molecular Plant Breeding;2006-02
3 DI Na,JIANG Shu-yuan,LI Guo-jing~ (School of Bioengineering,Inner Mongolia Agricultural University,Huhhot 010018,P.R.China);Several Critical Problems Need to be Resolved in Foreign Protein Expression using Plants[J];Biotechnology;2005-02
4 Wang Zhicheng 1,2 Yi Zili2 Pi Canhui2 (1 Dept. of Biology & Light Industry, Changsha University of Science & Technology,Changsha 410077; 2 Key Laboratory of Cell Engineering, Hunan Agricultural University, Changsha 410128);Progresses Problems and Strategies of Rice Breeding:Resistant to Insect & Disease via Transgenic Technique[J];Biotechnology Information;2004-03
5 DI Naa,b, JIANG Shu-yuana,b, WANG Rui-gangb,ca. College of Agronomy; b. Sugar Beet Physiological Research Institute; c. College of Bioengineering, Inner Mongolia Agricultural University, Hohhot 010018, China;Regulation of Foreign Gene Expression in Plant by Environmental, Physical and Chemical Factors[J];Letters in Biotechnology;2005-02
6 ZHANG Yan-juan1, YUAN Hong-xu1, ZHANG Jian-zhong1, GUO Jian-fu1, LI Bao-jian2 (1. Institute of Agriculture and Biotechnology, Zhanjiang Ocean University, Zhanjiang, Guangdong 524088, China; 2. Biotechnology Research Center of Zhongshan University, Guangzhou, Guangdong 510275, China);HETEROSIS AND COMBINING ABILITY OF RESISTANCE TO SHEATH BLIGHT OF TRANSGENIC RICE WITH EXTRA CHITINASE GENE[J];Journal of Southwest Agricultural University;2005-03
7 LI Jin-bin,YAO Chun-xin,XU Ming-hui*,LI Cheng-yun(Yunnan Academy of Agricultural Sciences,Agricultural Biological Technology Key Opening Laboratory of Yunnan,Yunnan Kunming 650223,China);Pyramiding lines of three foreign genes confer resistance to rice blast[J];Southwest China Journal of Agricultural Sciences;2007-01
8 WANG De-Zheng~1,WANG Shou-Hai~(1,①),WU Shuang~1,LI Cheng-Quan~1,JIAO De-Mao~2, LUO Yan-Chang~1,WANG Xiu-Feng~1,DU Shi-Yun~1 (1.Rice Research Institute,Anhui Academy of Agricultural Sciences,Hefei230031,China; 2.Institute of Agrobiological Genetics and Physiology,Jiangsu Academy of Agricultural Sciences,Nanjing210014,China);Inheritance and Expression of the Maize pepc Gene in Progenies of Transgenic Rice Bred by Crossing[J];Acta Genetica Sinica;2004-02
9 LI Ya-li~1, XU Ming-hui~1, YANG De~2, YANG Xiao-xi~2, ZHAO Feng-ping~1 ( 1. Biotechnology Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China; 2. College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China );Genetic Analysis of Foreign Lysozyme Gene in Rice[J];Journal of Yunnan Agricultural University;2004-02
10 LIU Fei-Hu1,*,LIANG Xue-Ni2,ZHOU Wei1,LIU Ming-Qiu1 1College of Life Science,2College of Adults Education,Yunnan University,Kunming 650091,China;Expression and Heredity of Exogenous SOD or APX Gene in Transgenic Tobacco (Nicotiana tabacum L.)[J];Plant Physiology Communications;2006-05
【Citations】
Chinese Journal Full-text Database 2 Hits
1 JianYuyu1  WuXingrong1  MoHaokue1  Chen Fengzhen1 Dong Chun2  Huang Ziran3(1 Genetic Engineering Laboratory, South China Agric. Univ., Guangzhou, 510642; 2 College of Natural Resources & Environment; 3 Dept. of Sericulture,South;TRANSFER OF CECROPIN B GENE INTO RICE (Oryza sativa L.) BY THE BIOLISTIC METHOD AND TRANSGENIC PROGENY LINE FOR RESISTANCE TO BACTERIAL BLIGHT[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1997-04
2 HUA Zhihua, WANG Xiaoling, X;Resistance of Cecropin B Gene Transformed Rice ( Oryza sativa ) and Progenies to Bacterial Blight ( Xanthomonas oryzae pv. oryzae )[J];CHINESE JOURNAL OF RICE SCIENCE;1999-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Zhi lan,YANG Min sheng,WANG Jin mao,LIANG Hai yong (Agricultural University of Hebei, Baoding 071000, China);Advances on genetic engineering breeding of poplar[J];Journal of Agricultural University of Hebei;2002-S1
2 WANG Yan1,LI Jiang-long1,PAN Yong-nian2,WANG Jia-zhen2(1.College of Life Science,Nanjing University,Nanjing 210093,China;2.Qingqing Grass Limited Company,Lishui 323000,China);Research progress in transgenic breeding of excellent warm-season turfgrass[J];Grassland and Turf;2007-04
3 Yao Xizhen Liao Sentai Xiao Gengsheng(The Sericultural Research Institute of Guangdong Academy of Agricultural Sciences,Guangzhou 510640);Discussion on the Industrialization of Comprehensive Development and Utilization of Silkworm and Mulberry Resources[J];Acta Sericologica Sinica;2001-04
4 HUANG Ziran~1 HUANG Yadong~1 WEN Liufa~1 ZHENG Qing~1 LIAO Fupin~1 HU Jianxiong~2 HUANG Guoqing~2 JIANG Lihua~2 HUANG Yongtong~2 LI Yunnan~3 LIANG Shuwa~3 PENG Zhongjian~3 DONG Jiaxi~3 XIA Fenggeng~3 CHEN Songbin~4 (~1South China Agricultural University,Guangzhou 510642,China;~2Shenzhen Yiping Bio.Engineering Co.Ltd.,Shenzhen 518032,China;~3Guangzhou Microbiology Institute,Guangzhou 510630,China;~4Huasang Bio.Engineering Co.Ltd.,Guangzhou 510520,China);Cecropin Biotechnology and its Application[J];Acta Sericologica Sinica;2005-04
5 LU Yongquan, WU Weiren(College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);Improvements in the technology for transformation of rice[J];;2003-01
6 C hen Dacheng,Zheng Qifa,and Hu Guibing (Department of Horticulture,South China Agricultural University,Guangzhou 510642) Huang Ziran (Department of Sericulture,SCAU);Research on the Genetic Transformation of Shatian Pummelo[J];SOUTH CHINA FRUITS;1999-04
7 Lin Chunjing1,2 Dong Yingshan1* Lin Xiufeng1 Wang Jichun3 Tian Wenzhong4 Chu Chengcai41 Center of Agri-Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 2 Institute of Genetics and Cytology, Northeast Normal University, Changchun, 130024; 3 Institute of Plant Protect, Jilin Academy of Agricultural Sciences, Gongzhuling, 136100; 4 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101;Primary Studies on Inheritance and Expression of Resistance to Rice Blast in Transgenic Rice[J];Molecular Plant Breeding;2006-02
8 SUN Jian-chang1,2,WANG Xing-sheng2,YANG Sheng-long2 (1.Northwest A & F University,Yangling,Shaanxi 712100,China; 2.Ningxia Academy of Agriculture and Forestry Sciences,Yongning,Ningxia 750105,China);Progress of research on salt-resistance in plants[J];Agricultural Research in the Arid Areas;2008-01
9 YU Hai-zhong,LIU Fa-bin(Department of Chemistry and Bioscience,Xiangfan University,Xiangfan 441053,China);The Exploitation and Ultilization Status of Insect Proteins[J];Chemistry & Bioengineering;2006-07
10 WANG Hong-quan1a,ZHANG Huai-yun2*,ZHANG Xue-wen1a,XIAO Tiao-yi1b,SU Jian-ming1b,TANG Xiang-bei1b(1.a.College of Biology Science and Technology;b.College of Animal Science and Technology,HNAU,Changsha 410128,China;2.Central South Forestry University,Changsha 410004,China);The Study on the Expression of Hu-IFN-α Gene in F_1 Generation of Transgenic Grass Carp[J];Journal of Hunan Agricultural University(Natural Sciences);2006-01
China Proceedings of conference Full-text Database 2 Hits
1 ;椪柑愈伤组织的遗传转化及转化体的再生[A];[C];2006
2 Huang Ziran Huang Yadong Wen Liufa Zheng Qing Liao Fuping Hu Jianxiong Huang Guoqing Jiang Lihua Huang Yongtong Li Yunnan LiangShuwa Pang Zhongjian Dong Jiaxi Xia Fenggeng Chen Songbin South China Agricultural University Guangzhou 510642; ShenZhen Yiping Bio Engineering Co Ltd, Shenzhen 518032; Guangzhou Microbiology Institute, Guangzhou 510630; HuaSang Bio Engineering Co Ltd, Guangzhou 510520.;Cecropin biotechnology and its application[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 WANG Jia-bao,WANG Liu-ming,SHEN Fa-fu,ZHANG Xue-kun,YANG Jing,LIU Ren-zhong (Shangdong Cotton Research Center,Jinan 250100,China);Effect of environment elements on Bt-protein content in transgenic Bt cotton[J];Shandong Agricultural Sciences;2000-06
2 LI Ru-zhong 1,SHEN Fa-fu 2,WANG Zong-wen 1,WANG Jing-hui 1,LIU Cheng-yun 1,SHEN Gui-fang 1 (1 Shandong Cotton Research Center, Jinan 250100,China;2 Shandong Agricultural University, Taian 271018,China);Temporal and spatial dynamics of Bt gene expression in Bt transgenic cotton[J];Shandong Agricultural Sciences;2002-02
3 Gao Junshan et al (Anhui Agricultural University,Hefei 230036);Summary of the Techniques and Methods of Plant Gene Transformafion[J];Journal of Anhui Agricultural Sciences;2003-05
4 ZHOU Dong sheng 1,WU Zhen ting 1,WANG Xue lin 1,NI Chun geng 1, ZHENG Hou jin 2,XIA Jing 2 (1 Department of Plant Protection, Anhui Agricultural University,Hefei 230036; 2 The General Seeds Company of Anhui Province);Influence of Fertilization and Environmental Temperature on the Resistance of Bt Transgenic Cotton to Cotton Bollworm[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-04
5 YU Zhihua CHEN Minyong\ GU Hongya\ PAN Naisui\ CHEN Zhangliang (College of Life Sciences, Peking University, Beijing, 100871) LI\ Yan (Dandong Academy of Agriculture Sciences, Liaoning, 118109);Upland Rice Transformation[J];ACTA SCICENTIARUM NATURALUM UNIVERSITIS PEKINESIS;1996-04
6 ZHANG Guo-hua 1 , CONG Ai-ri 2, XU Guo-bin 1△ , SUN Li-ying 1, YAN Yan 1, XIA Tie-an 1(1. Department of Clinical Laboratory Medicine,Peking University First Hospital,Beijing 100034, China; 2. Shandong Weihai Wendeng Central Hospital);Isolation and identification of Comamonas testosteroni: cloning and overexpression of 3α-hydroxysteroid dehydrogenase in E.coli.[J];Journal of Peking University(Health Sciences);2005-02
7 CUI Dong-bo, ZHENG Yan-jie, WANG Yun-ji, ZHANG ling-hua(School of Biological & Foodstuff Engineering, Dalian Institute of Light Industry, Dalian 116034,China);Purification of antibacterial peptides from earthworm[J];Journal of Dalian Institute of Light Industry;2004-04
8 MA De hua,PANG Jin an,HUO Zhen rong,LI Shu ju (Tianjin Cucumber Research Institute,Tianjin 300192,China);Effect of high temperature on membrane lipid peroxidation in leaf of cucumber seedling[J];ACTA BOTANICA BOREALI-OCCIDENTALIA SINICA;2000-01
9 MA De hua 1,SUN Qi xin 2 (1 Tianjin Cucumber Research Institute,Tianjin 300192,China;2 Agricultural Department,Chinese Agricultural University,Beijing 100093,China);Effects of temperature stress on membrane protective system of cucumber seedlings[J];Acta Botanica Boreali-occidentalia Sinica;2001-04
10 LIU Hong-sheng,SONG Qiu-hua,LI Feng-min(State Key Laboratory of Arid Agroecology,Lanzhou University,Lanzhou 730000,China);The roles of root exudation on rhizosphere nutrient and rhizosphere microorganisms[J];Acta Botanica Boreali-occidentalia Sinica;2002-03
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 LAI Jian-xun et al (College of Agronomy, South China University of Tropical Agriculture, Danzhou, Hainan 571737);cDNA Cloning and Analysis of Ascorbate Peroxidase Gene from Pericarp of Litchi[J];Journal of Anhui Agricultural Sciences;2007-26
2 DENG Qi-ming et al(Rice Research Institution,Sichuan Agricultural University,Chengdu,Sichuan 611130);Study Progress on Rice Blast Resistance Gene Researches and Its Application in Blast Resistance Breeding[J];Journal of Anhui Agricultural Sciences;2009-04
3 Li Xia* Jiao DemaoInstitute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014;Transgenic Rice Overexpressing C_4 Photosynthetic Genes and Their Application in Breeding[J];Molecular Plant Breeding;2005-04
4 Jin Wanmei1,2* Pan Qinghua2 Yin Shuping2 Dong Jing2 Jiang Lijie2 Yang Li2 Chen Qinghua2 Zhao Jianbo2 1 College of Agricultural and Biotechnology Science, China Agricultural Universi ty, Beijing, 100094; 2 Institute of Forestry and Pomology, Beijing Academy of Ag riculture and Forestry Science, Beijing 100093;Progress of the Genetic Stability and Breeding Behavior of Foreign Gene in Genetically Modified Plants[J];Molecular Plant Breeding;2005-06
5 Zhang Kaizheng1,2 Li Ping1,2* Li Na3 Xiang Xunchao1, 2, 4 1 Rice Research Institute, Sichuan Agricultural University, Wenjiang, 611130; 2 Key Laboratory of Southwest Crop Genetic Resource and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, 625014; 3 Seed Multiplication Farm of Fushun County in Sichuan, Fushun, 643200; 4 Southwest University of Science and Technology, Mianyang, 621000;Research Progress in Germplasm,Heredity and Breeding of Resistance to Rice Sheath Blight[J];Molecular Plant Breeding;2006-05
6 Liu Yan1,3 Hu Yuanlei2 Dong Jing1 Tian Zihua3 Chen Meixiang1 Jin Wanmei1 1 Institute of Forestry and Pomology, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100093; 2 The College of Life Science, Peking University, Beijing, 1000871; 3 Department of Agriculture of Inner Mongolia Agricultural University, Hohhot, 010019;Study on CBF1 Gene Transferred from Transgenic Strawberry to Nontransgenic Strawberry[J];Molecular Plant Breeding;2007-03
7 He Longfei Mo Changming Li Chuangzhen Lu Sheng'an Wang Aiqin Yi Xiaoping College of Agronomy, Guangxi University, Nanning, 530004;Effects of Transgenic hpt and sck on Agronomic Characteristics of Rice Male Sterility Line and its Combinations[J];Molecular Plant Breeding;2008-02
8 WANG Chao1,2,LI Xia1(1.Institute of Foods Crop,Jiangsu Academy of Agriculture Sciences,Nanjing 210014,China;2.College of Life Science,Nanjing Agricultural University,Nanjing 210095,China);Comparison of Micro-structure Between Transgenic Rice of PEPC Gene from Maize and Wild Type under the Strong Light[J];Acta Agriculturae Boreali-Sinica;2007-04
9 LIU He1,XIE Ling-qin1,ZHAO Jian-jun2(1.College of Agronomy,Agricultural University of Hebei,Baoding 071001,China;2.College of Horticulture,Agricultural University of Hebei,Baoding 071001,China);Reviews on Plant Resistance Genes and the Related Resistant Mechanisms[J];Acta Agriculturae Boreali-Sinica;2007-S2
10 WEN Jia-wei,REN Jin-ping,GUO Xiao-li,LI Li,LIU Xiao-mei,SUN Hui,WANG Ji-chun(Plant Protect Research Institute of Jilin Academy of Agricultural Sciences,Gongzhuling,Jilin 136100);Progress of Study on Rice Blast[J];Heilongjiang Agricultural Sciences;2009-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved