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《Journal of South China Agricultural University》 2009-01
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Active Ingredients of Seed Extracts of Clausena lansium and Nematocidal Activity Against Bursaphelenchus xylophilus

MA Fu-ning,WAN Shu-qing,LIU Xu-ming,ZHAO Feng(Key Lab of Natural Pesticide and Chemical Biology,Ministry of Education,Lab of Insect Toxicology,South China Agricultural University,Guangzhou 510642,China)  
Nematocidal activity of methanol extracts from the different parts of Clausena lansium was determined by dipping method.The active ingredients were isolated and identified on the basis of active-directed fractionation with column chromatography,thin layer chromatography(TLC),recrystallization and nuclear magnetic resonance(NMR).The results showed that the most effective extract against Bursaphelenchus xylosphilus was seed methanol extract,which gave 100% mortality after 72 h treatment at the mass concentration of 1 mg/mL.The active ingredients were concentrated in petroleum ether fraction and a yellow crystal was obtained and purified.It was identified as Lansiumamide B(N-methyl-N-cis-styryl-cinnamamide) on the basis of NMR data.The bioassay results revealed that LC50 values of Lansiumamide B against B.xylophilus were 8.38,6.36 and 5.38 mg/L after 24,48 and 72 h treatment,respectively.The results indicated that Lansiumamide B was effective against B.xylophilus.
【Fund】: 广东省自然科学基金(990703 010319);; 天然农药及化学生物学教育部重点实验室基金(2005)
【CateGory Index】: S769
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