Study on the Scavenging Hydroxyl Radical of the Extracts from Smilacina japonica

ZHAO Shu-jie1,2,HAN Zhong-ming2,LI Yan-ying2,YANG Li-min2(1 College of Resources and Environment,Jilin Agricultural University,Changchun 130118,China;2 College of Chinese Medicinal Materials,Jilin Agricultural University,Changchun 130118,China)  

The extractions from the rhizomes of Smilacina japonica were obtained through hot water extraction,Soxlet's extraction and macroprorous adsorbing resin.Obtained water extraction,alcohol extraction,water solution of alcohol extraction from the rhizomes of Smilacina japonica,and Ⅰ,Ⅱ,Ⅲ,Ⅳ four parts of matter were isolated from alcohol extract by macroporous adsorbing resin method in turn with 30%,50%,70%,90% ethanol elution,and five flavonoids,3-methoxy-8-methylquercetin(A),8-methylluteolin(B),3′-methoxyluteolin(C),luteolin(D) and quercetin(E) were isolated from the Ⅲ.The method of phenanthroline-Fe2+-H2O2 reaction system was used to measure scavenging effect to hydroxyl radical(HO·).The results showed that all extracts of Smilacina japonica had ability to scavenge hydroxyl radical,the scavenging ability was 23.30%-98.07% except alcohol extraction,the scavenging ability of five compounds were stronger even than that of Vit C in dose-dependant manner.Therefore,plant of Smilacina japonica has good antioxidant active ingredient.

【Key Words】： Smilacina japonica extraction flavonoid hydroxyl radical antioxidation
【Fund】： 吉林省科技发展计划重大项目(20075022)
【CateGory Index】： R285

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