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《Journal of Hunan Agricultural University》 2004-04
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Identification of Rapid Marker Linked to a Restorer Gene for Cytoplasmic Male Sterility in Pepper

TANG Dong-ying1,2,ZOU Xue-xiao1,LIU Zhi-min3,MA Yan-qing1,ZHANG Zhu-qing1,DAI Xiong-ze1 (1.Institute of Vegetables,Hunan Academy of Agricultural Sciences,Changsha 410125,PRC;2.Biotechnology Institute of Hunan University,Changsha 410082,PRC;3.College of Horticultural and Landscape,HNAU,Changsha 410128,PRC )  
With F2 segreganting population of 9704A×8001,molecular markers linked to a fertility restorer (Rf)gene for cytoplasmic male sterility in pepper(Capsicum annuum L.)were screened by bulked segregant analysis using RAPD-PCR.A RAPD fragment specific to the bulked DNA samples from male-fertile F2 plants were identified.Further linkage analysis performed with 120 individuals in the F2 segregating population showed that the RAPD marker OPL09-763 linked to the Rf gene in restorer 8001 and positioned 4.18 cM from the Rf gene.The exchange value between the marker and Rf gene was 4.17%.On the basis of sequencing,the homology was analyzed between the OPL09-763 and other homologous region in GenBank.
【Fund】: 国家“863”计划资助项目(2002AA244021)
【CateGory Index】: S641.303.53
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