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《Journal of Hunan Agricultural University(Natural Sciences)》 2007-02
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Genetic diversity among five geographical populations of Chilo suppressalis based on RAPD

XUE Jin1,2,SU Jian-wei2,LI Jia-wen1,CHEN Qiu-fang1(1.College of Bio-Safety Science and Technology,HNAU,Changsha 410128,China;2.State Key Laboratory of Integrated Management of Pest Insect and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing 100080,China)  
Five geographical populations of Chilo suppressalis are detected by RAPD.Twenty-nine primers are screened from 50 random primers and amplified 220 bands(from 200 bp to 3 000 bp).The number of obtaining segments of individual primer is between 2~15 with an average 7.59.97 of these bands are polymorphic bands.Polymorphisms,genetic similarity indices and genetic distance were calculated.The results of the study are as below:(1) The genetic diversity among five geographical populations could be distinguished from the polymorphisms.Polymorphisms from Quanzhou,Guanxi are the smallest while that from Liuhe,Jilin are the biggest;(2) The genetic comparability of five geographical populations varies 0.551 88 to 0.849 00 and the range of genetic distance is from 0.121 42 to 0.448 12 and the average was 0.223 06.The genetic distance between the two populations from Lujiang,Anhui and Wuhan,Hubei is the smallest among five populations.However,the genetic distance between the two populations from Liuhe,Jilin and Quanzhou,Guangxi is the biggest.
【Fund】: 中国科学院知识创新工程前沿项目(KSCX-IOZ-04);; 湖南农业大学青年科学基金项目(06QN09)
【CateGory Index】: S435.112.1
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