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《Journal of Hunan Agricultural University(Natural Sciences)》 2007-04
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Establishment of in vitro propagation system with high-efficiency based on three cultivars of Dracaena fragrans

YI Qing1,HE Ye-hua2,LIU Song-song3,Lü Chang-ping1,LIU He-ping2,LIN Shun-quan2,ZHU Jian-yun3,YE Yong-chang3(1.College of Horticulture and Landscape,HNAU,Changsha 410128,China;2.South China Agriculture Guangzhou 510642,China;3.Forestry Institute of Dongguan,Dongguan,Guangdong 523006,China)  
Taking the stem from three varieties of Dracaena fragrans as a material,the effect of the factors such as the plant growth regulatorst,he different basal culture mediums,ilver nitrate,and the cultivation medium upon tissue culture regeneration were investigated.The results showed that the axillary buds germinated,at the same time stem tissue differentiation around axillary buds substratum was initiated with MS+3.0 mg/L BA+0.3 mg/L IAA+2.0 mg/L AD;then one generation were raised on MS+3.0 mg/L BA+0.3 mg/L IAA,in which 5~6 adventitious buds split up from each explants tall about 1mm equally;the proliferation rate of adventitious bud reached is over 390.00% for MS+1.0 mg/L BA+0.1 mg/L IAA+3.0 mg/L AgNO3.One generation of the adventitious buds bigger than 1.2 cm were raised as the strong sprouts in MS+0.05 mg/L BA+0.05 mg/L IAA+1.0%AC,the bud increased approximately above 1.5 times stronger in coarseness with average height about 3.5 cm;The sprout higher than 3 cm were transitted to 1/2MS+0.5 mg/L IBA to induce rooting;Finally the seedling grows were transitted to the combination with the coconut tree rice polishings∶Marly soil∶On the pearlite(1∶1∶1),after 45 days,the height of the three seedlings reached above 15,16,10 cm respectively.Over two years of test,a highly effective and stable tissue culture system of Dracaena fragrans was established,its initiation rates,bud differentiation rater,ooting rates,and survival rates was above 98% stably.Three varieties display the similar rule in the start,in the processes of the bud differentiation,multiplication,takeing root,transplanting and so on in particular Dracaena fragrans cv.massangeana and the ordinary Dracaena fragrans are quite close,but there was a higher seedlings and a low growth potential difference in Lindenii.It is vital to reduce the variation of Dracaena fragrans cv.massangeana and Dracaena fragrans cv.lindenii by preventing the differentiation from forming callus as well as the reduction or the prevention of callus redifferentiation.
【Fund】: 广东省科技重点项目(2006B20101003)
【CateGory Index】: S682.36
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