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Comparative analysis of genetic diversity in landraces of maize (Zea mays L.) from Sichuan and Chongqing

YAO Qi-lun1,2,XU Jiang2,XU Dong-mei2,CHEN Xiu-juan2(1.Department of Life Science,Yangtze Normal College,Fuling,Chongqing 408003,China;2.Maize Research Institute,Sichuan Agricultural University,Yaan,Sichuan 625014,China)  
The genetic diversity in landraces of 30 maize 15 from Sichuan and 15 from Chongqing,was tested by using 42 microsatellite(SSR) loci.256 and 248 polymorphic alleles were detected,and the average number of alleles were 6.1 and 5.9,respectively,and the average polymorphism information content(PIC) were 0.76 and 0.73,respectively.Clustering analysis separated the landraces from Sichuan and Chongqing into 4 and 3 groups,respecively.In addition,the genetic diversity was greater in Sichuan than that in Chongqing.The results based on the gentic diversity and the genetic structure showed that there was an obvious genetic differentiation from Hardy-Weinberg expectation in the landraces from both Sichuan and Chongqing and a considerable genetic variation was revealed within rather than among landraces.The genetic diversity on the basis of SSRs provides a guide for maize breeders to conserve and utilize the germplasm.
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