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《Journal of Hunan Agricultural University(Natural Sciences)》 2009-03
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DNA extraction and optimization of SRAP reaction system in tobacco

XU Jian-hua1,YANG Hong-qi1,YANG Cheng2,ZHOU Ji-heng1,DUAN Feng-yun2,CHEN Xin-bo1,ZHAN You-guo1,ZHAO Shi-hao1(1.Key Laboratory of Tobacco Science and Healthy,HNAU,Changsha 410128,China;2.Technology Center Tobacco Dali Corp,Dali,Yunnan 671000,China)  
Method of DNA extraction and factors influencing SRAP analysis were studied using eight tobacco cultivars.The results showed that,improved method of CTAB contributed to a better extraction of genomic DNA of tobacco.A reliable PCR reaction system for detecting SRAP was developed,each 20 μL PCR reaction mixture consisted of 40 ng of genomic DNA,0.25 mmol/L of dNTPs,1.5 mmol/L of Mg2+,0.2 μmol/L of primer and 1.5 unit of Taq polymerase,every amplification out of the eight cultivars was effective,clear and their differences were significant.
【Fund】: 云南省大理自治州烟草公司项目(20060678)
【CateGory Index】: S572.024
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