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《Hunan Agricultural Sciences》 2006-04
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Influence Factors of Microbial Protoplast Fusion Technology

ZHENG Zhong-yi~1,XIE Da-ping~1,TAN Zhou-jin~2,XIAO Ke-yu~2,LI Yu-hong~1 (1.College of BioScience and Technology,Hunan Agricultural University,Changsha 410128,PRC;2.Hunan Agricultural University,Changsha 410128,PRC)  
The protoplast fusion technology is an effective ways of microbial breeding,including main processes of protoplast preparation,protoplast fusion,protoplast regeneration,fusion separation.An review was given on the influence factors on composition and quomodo of medium culture,age of bacterium growing,kinds and concentration of enzyme,stabilizer,molecular weight and concentration of PEG.The summarize about the new development and appliance of the technolegy of microbe protoplast fusion was given in the article.
【Fund】: 湖南省教育厅青年基金(05B029)
【CateGory Index】: Q93-33
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