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《Hunan Agricultural Sciences》 2008-01
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Research on the Electrofusion Conditions of Protoplasts Between 'Jigu 5' and 'Shuyou1'

LUO Ying-lan,LI Yuan-wen,XIA Zhi-lan(Edible Fungi Institute,Hunan Agricultural University,Changsha 410128,PRC)  
The electrofusion conditions of protoplasts between "Jigu 5" and "Shuyou1" were studied.It showed that the fusion frequency of protoplasts reached 21% with the following parameters:alternate current(AC) 1 500 kHz,300 V/cm,direct current(DC) 2.5 KV/cm,DC pulse width(PW) 60 μs,number of DC pulse 4~5 times,mannitol 10% and CaCl2 0.4 mmol/L in electrofusion solution.
【Fund】: 湖南省科技厅下达的科技攻关项目(KTY2004)
【CateGory Index】: S646
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