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《Hunan Agricultural Sciences》 2008-02
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Tissue Culture of Taxus Chinensis Var. Mairei

TANG Dao-fang1,LIU De-hua1,JIANG Li-wen1,WANG Shu-zhi1,LIU Li1,XIANG Bo-wen1,XIANG Qin-zeng2,ZHANG Li-xia2(1.College of Horticultural and Landscape,HNAU,Changsha 410128,PRC;2.College of Horticulture,Shandong Agricultural University,Taian 271018,PRC)  
The regeneration system of Taxus chinensis Var.Mairei was studied using stems with axillary buds as explants.The results showed that the optimal explants were young twigs in the growing state.The optimal medium of axillary buds germination and growth was DCR+IBA 0.1 mg/L+BA 0.05 mg/L.The optimal medium of roots was White+IBA 0.2 mg/L.Trans-plant seedlings into pan with nutrition when the length of root was 0.5 cm,temperature 25 ℃,humidity above 75%,gradually increased the intensity of illumination in 30 days,the plant survival rate reached to 90.1%.
【Fund】: 湖南省教育厅项目(63020152)
【CateGory Index】: S791.49
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