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《Hunan Agricultural Sciences》 2010-03
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Screening and Cloning of Differentially Expressed Genes from Gonad of Monopterus albus

QU Xian-cheng1,CHENG Cui1,SHANG Xiao-li1,QU Xue-wei2,ZHANG Kai-yue1,ZHANG Yong1(1.College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,PRC;2.Muping Fisheries Technical Service Center,Yantai 264100,PRC)  
Using annealing control primer(ACP) based differential display reverse transcription polymerase chain reaction(ACP-DDRT-PCR) to screen and clone differentially expressed genes(DEGs) from gonad in ovary at stage IV and ovotestis stages of Monopterus albus.Using two arbitrary ACP primers to identify two DEGs,and then these two were cloned and sequence analyzed to obtained parts of cDNA sequences of these two genes,and the length of them were 408bp and 421 bp,respectively.The homologous analysis revealed that one of the genes has highly homologous to one of the genes in cDNA library of yellow perch ovarian(GeneBank No.GO657149.1);while the other gene has nonhomologous sequence,which is a new reported gene.Further Semi-quantitative RT-PCR test results revealed that there was a significantly difference between expressions of these two genes in ovary at stage IV and ovotestis of Monopterus albus.So it supposed that these two genes play important roles in the gonadal development and sex-reversal of Monopterus albus.
【Fund】: 上海市重点学科建设项目资助(S30701)
【CateGory Index】: S917.4
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