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《农业科学与技术(英文版)》 2008-02
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Histological Study on Soybean Somatic Embryogenesis

ZHAO Gui-lan1,YANG Xiang-dong1,GUO Dong-quan1,HU Zan-min21. Biotechnology Research Center,Jilin Academy of Agricultural Sciences,Changchun 130124; 2.Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101  
Soybean somatic cell could induce the development of embryoid which was similar to embryo morphologically and structurally. Somatic embryogenesis system of soybean was used to conduct genetic transformation of soybean because of its several advantages such as higher transformational efficiency,beetter synchronism and fewer plant chimeras among transgenic plants. After infected with agrobacterium tumefaciens,the initiation,differentiation and development of young cotyledon embryogenic cell of soybean which was cultured on selective culture medium with kanamycin were investigated through histological study. The result showed that somatic embryo was differentiated in non-bud differentiation way. The embryogenic cells were differentiated from epidermis of explant or cells in 1 layer or 2 layers,with the division of embryogenic cells and degradation and disorganization of surrounding cells,the embryogenic cells would form embryoid with analogous suspensor structure.Later,globular embryoid would extrude from epidermis then developed into heart-shape embryo.The experiment was expected to provide theoretical reference for the construction of high transformational system of using plant somatic embryogenesis induced by young cotyledon of soybean.
【Fund】: the National Natural Science Foundation of China (C02020504);; the Scientific and Techrological Developing Scheme of Jilin Province (20050217-2;20060204);; the national 863 project (2006AA100104-17)~~
【CateGory Index】: S565.1
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