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《农业科学与技术(英文版)》 2013-04
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Analysis of Monosaccharide Composition of Pu-erh Tea Polysaccaride by Pre-column Derivatization HPLC

Wei GUO,Binxing ZHOU *,Ling LUO,Yang LI,Jie CHAI,Chao YANG,Cunqiang MA Pu-erh Tea College,Yunnan Agricultural University,Kunming 650201,China  
[Objective] This study aimed to establish a pre-column derivatization HPLC method for the identification and analysis of monosaccharide composition of Pu-erh tea polysaccharide.[Method] Pu-erh tea polysaccharide was extracted using the water extraction method,further isolated and purified by DEAE cellulose-52 columns.The obtained tea polysaccharide and four components TPS 1,TPS 2,TPS 3 and TPS 4 were first derived by 1-phenyl-3-methyl-5-pyrazolone(PMP),and then the PMP derivatives of monosaccharide were analyzed by high performance liquid chromatography(HPLC).[Result] Pu-erh tea polysaccharide contained eight kinds of monosaccharides(mannose,rhamnose,glucuronic acid,galacturonic acid,glucose,galactose,arabinose,fucose),without xylose;so it was the same with TPS 1 ;each of TPS 2,TPS 3 and TPS 4 contained seven monosaccharides,while no fucose.[Conclusion] This method is simplified and rapid,which can be used to determine the monosaccharide composition of Pu-erh tea polysaccharide and monosaccharide content.
【Fund】: Supported by National Key Technologies R & D Program(2007BAD58B03)~~
【CateGory Index】: TS272.7;O657.72
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