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《Journal of Henan Agricultural Sciences》 2004-08
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Studies on the Technique System of Tissue Culture and Rapid Propagation of Jojoba I. Selection of Basic Medium and Culture Condition

XU Jin 1,2 ,WANG Yu zhen 1,LUO Jing lan 1,LIU Xiao jing 1 (1 Center for Agricultural Resources Research,Institute of Genetics and Developmental Biology, Chinese Academy of Sciences,Shijiazhuang 050021,China;2 Graduate School of the Chinese Academy of Sciences)  
The key techniques including explants disinfection, basic medium, suitable explant and culture condition were studied.By culturing the stems of Jojoba in vitro,the results showed as that the success percentage of disinfection reached 99% when the explants were treated by 1∶1 mixture of 0.1% HgCl 2 and 2% NaClO for eight minutes,the modified MS was suitable for Jojoba micropropagation.The optimal explants were stems with double axillary buds.All the cultures were kept under the temperature of 25-28 ℃,light intensity 3000 lx,light time for 12-14 h/d,and pH 5.5-6.0.
【Fund】: 河北省国际科技合作计划项目资助 ( 0 3390 16 2D)
【CateGory Index】: S792
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