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《Journal of Henan Agricultural Sciences》 2006-01
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Construction of cDNA Library and Cloning of Globulin Gene from Wild Soybean(Glycine soja) Seeds

WANG Qing-lian,SHI Ming-wang(Henan Institute of Science and Technology,Xinxiang 453003,China)  
The cDNA library containing 9.594×10~5 clones was established.Using the ripe seeds of high-quality wild soybeans,the percentage of recombination was as high as 93.7%,and PCR results showed that the average size of inserts was larger than 1000bp,217 expressed sequence taqs were gained from 252 clones,randomly from the library selected by sequencing at the 3′ end.Sequencing length of fragments was with over 500bp.99% globulin cDNA sequence was similar between Glycine soja and Glycine max.
【Fund】: 河南省杰出人才创新基金项目(0321001400)
【CateGory Index】: S565.1
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