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《Journal of Henan Agricultural Sciences》 2010-05
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RAPD Analysis of the Wild Mice from Henan and Inbred Strains of Mouse

LEI Jian1,HUA Xin-xin2,ZHOU Xin2,CHAI Bao-guo2,ZHAO Wei-dong1,ZHENG Zhen-yu1,2(1.College of Animal Husbandry and Veterinary Medicine,Henan Agricultural University,Zhengzhou 450002,China;2.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,China)  
The RAPD analysis of Henan wild mouse and other four inbred strains of mouse were shown in this study.A total of 304 polymorphic fragments were achieved from 380 amplified fragments using 36 polymorphic primers which were selected from 120 random primers.Several specific bands possessed by wild mouse reflected that the RAPD polymorphisms exist among the four inbred strains of mouse and wild mice.The results of statistical analysis of RAPD bands showed that the genetic similarity between wild mice and PWK is 0.00924,which indicated that wild mice had the closest relationship to PWK.However,the genetic similarity between wild mice and DDK is 0.01130,which showed the wild mice were clearly apart from DDK.These results indicated that there were genetic similarities and variations between wild mouse and inbred strains of mouse.Thus,as a molecular marker,RAPD can be used to detect the variations and distinguish the mouse strains.
【Fund】: 转基因生物新品种培育科技重大专项项目(2009ZX08009-157B);; 河南省科技创新杰出人才计划项目(094200510010)
【CateGory Index】: Q953
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