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《Journal of Henan Agricultural Sciences》 2012-06
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Development and Characterization of Monoclonal Antibody against GP5 Protein of Porcine Reproduction and Respiratory Syndrome Virus

WANG Zhi-hong1,2,ZHANG Gai-ping2,GUO Jun-qing2,LI Qing-mei2,WANG Yin-biao2,CHEN Wen2,WANG Ai-ping1 (1.Department of Bioengineering,Zhengzhou University,Zhengzhou 450001,China; 2.Key Laboratory of Animal Immunology of the Ministry of Agriculture/Henan Provincial Key Laboratory of Animal Immunology,Henan Academy of Agriculture Sciences,Zhengzhou 450002,China)  
In order to prepare monoclonal antibodies(mAbs) against porcine reproductive and respiratory syndrome virus(PPRSV),the recombination GP5 protein of PRRSV was used to immunize BALB/c mouse and the spleen cells were fused with NS0 cells.Three hybridoma cell lines producing mAbs specific to the GP5 protein,designated as 4B7,2C5 and 2E4,were obtained after identified by indirect and inhibit ELISAs followed by limiting dilution subcloning.All of the mAbs were shown to react specifically with the Marc-145 cells infected with PRRSV BJ-4 strain in indirect immunofluorescence assay(IFA) andimmunoperoxidase monolayer assays(IPMA).Ig subclass of the mAbs was determined as IgG1,and the ELISA titers of cell supernatant and ascites were 1∶256 and 1∶51 200,respectively.Western-blotanalysis showed that the mAbs were specific to the GP5 protein of PRRSV.
【Fund】: 国家自然科学基金面上项目(31072122)
【CateGory Index】: S858.28
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