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《Journal of Henan Normal University(Natural Science)》 2009-03
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Optimization of Amplification and Construction of DNA Fingerprinting for Rehmannia Glutinosa Libosh. f. Hueichingensis (Chao et Schih) Hsiao Based on SRAP

GU Feng-ping,ZHOU Chun-e,LU Shu-xia,YAO Huan-ling,WANG Fang,DUAN Hong-ying,ZHOU Yanq-qing(College of Life Sciences,Henan Normal University,Xinxiang 453007,China)  
In the present study,SRAP-PCR parameters are optimized using Rehmannia Glutinosa Libosh.f.Hueichingensis(Chao et Schih) Hsiao,DNA as template.SRAP fingerprinting for different cultivars of Rehmannia glutinosa L.is contructed.The optional parameters for 25 μL PCR reaction volume are as follows: Mg2+2.5 mmol ·L-1,dNTPs 0.30 mmol·L-1,primer0.36 μmol·L-1,DNA polymerase 2.0 U,and template DNA 20 ng.SRAP-PCR amplification by which SRAP DNA fingerprinting for different cultivars of Rehmannia glutinosa L.is successfully constructed,This is the foundation for the cultivar identification,genetic diversity evaluationmarker-assisted selection breeding and quality assessment.
【Fund】: 国家高技术研究发展计划(863)项目子课题(2006AA100109)资助
【CateGory Index】: S567.234
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