THE EFFECT OF ~(60)Co γ RAY IRRADIATION ON THE GROWTH AND REGENERATION OF Cymbidium sinense RHIZOME
FU Xue\|lin 1\ ZHANG Zhi\|sheng 1\ HE Ping 2\ OU Xiu\|juan 1\ HE Qiong\|ying 1 (1 Agronomy Department of South China Agricultural University, Guangzhou, Guangdong prov.\ 510642; 2 Biotechnology College of South China Agricultural University, Guan
The effect of 60 Co γ\|ray irradiation on growth of Cymbidium sinense rhizome and shoot regeneration were studied. The results showed that irradiation with low dose (2,5 and 8Gy) promoted the growth of Cymbidium sinense rhizome, while high dose(≥10Gy)inhibited the growth. Shoot defferentiation of rhizome was not affected by irradiation of low dose. Biochemical analyses indicated that the soluble protein content of rhizome irradiated with low dose was higher than that of CK, irradiated with 2 and 5Gy seemed to reduce the activity of peroxidase, while irradiated with 8Gy increased the activity of peroxidase.