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《Journal of Nuclear Agricultural Sciences》 2008-01
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EFFECT OF ~(60)Co γ-rays IRRADIATION ON GROWTH AND ANTIOXIDANT ENZYME ACTIVITIES OF RHIZOMES IN Cymbidium goeringii

LIU Lu-lu~1 CHAI Ming-liang~1 XU Xiao-wei~2 LI Fang~1(1.Department of Horticulture,Zhejiang University,Hangzhou,Zhejiang 310029; 2.Subtropical Crop Research Institute,Wenzhou,Zhejiang 325005)  
The rhizomes of Cymbidium goeringii were cut into tip,middle and basal segments with a length of about 1 cm,and the segments were irradiated with()~(60)Co γ-rays at doses of 0,5,10,20,30 and 50 Gy,respectively. The growth characters,the activities of antioxidant enzymes,including superoxide dismutase(SOD),catalase(CAT) and guaiacol peroxidase(G-POD),and peroxidase isozyme of the were measured.The results showed that the segments irradiated at the dose of 30 and 50 Gy could survive,but couldn't form new rhizomes and the growth was almost stagnated.With the increase of the doses,the SOD and CAT activities of the rhizome were increased first,then significantly decreased,but the reverse effect was observed in G-POD activities,which was decreased first,then increased.Combining both the effect results on the growth characters,on activity changes of peroxidase isozyme and the changes of the anti-oxidant enzymes after irradiation,it was concluded that the appropriate doses of()~(60)Co γ-rays irradiation for mutation breeding of Cymbidium goeringii should be in the rage of 20 to 30 Gy,and the optimal part of the rhizome seems to be the tip segment.
【CateGory Index】: S682.31
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