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《Journal of Nuclear Agricultural Sciences》 2008-01
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ZHANG Quan~1 HUANG Jian-zhong~1 YANG Zheng-min~2 ZHU Qi-song~1 YE Qing-fu~1 L Long~2 XU Bu-jin~1 CHEN Zi-yuan~1(1.Institute of Nuclear-Agricultural Sciences/Key Laboratory of Nuclear Agricultural Sciences of Ministry of Agriculture and Zhejiang Province,Zhejiang University,Hangzhou,Zhejiang 310029;2.Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032)  
Microsomes were prepared from etiolated wheat seedlings pretreated with 5%(v/v) ethanol and 10mmol/L Pyribambenz-propyl for 24h at 25℃ in a dark and reacted with [C ring-U-()~(14)C] pyribambenz-propyl).Radioactive labeled compounds were extracted from the reaction solution with ethyl acetate,separated and determined by HPLC-LSC,and then identified by HPLC-MS(ESI).Three major metabolites(Y1,Y2 and Y3),presumably related to Smiles rearrangement,indicated that Pyribambenz-propyl underwent hydroxylation in wheat microsomes and the in vitro system might also facilitate isomerization of Pyribambenz-propyl.Further studies are required to clarify whether rearrangement reactions of Pyribambenz-propyl is ready to occur within plant cells and whether these Smiles rearrangement-related metabolites are involved in herbicidal activities and/or detoxification of the(pro-)herbicide.
【Fund】: 国家自然科学基金面上项目(10575086);; 国家自然科学基金重点项目(20632070);; 中国科学院知识创新工程重要方向项目(KGCX3-SYW-203-03);; 浙江省科技厅院士基金
【CateGory Index】: S482.4
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