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《Journal of Nuclear Agricultural Sciences》 2008-02
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IDENTIFICATION OF MORPHOLOGY AND CYTOLOGY IN WHEAT HAPLOID THROUGH WHEAT×MAIZE

CAI Hua1 MA Chuan-xi1 QIAO Yu-qiang1(1.College of Agronomy,Anhui Agricultural University,Hefei,Anhui 230036)  
In order to identify the haploidy of hybrid progeny through wheat×maize,phenotype and chromosome number of hybrid embryo and plants produced by embryo rescue were analyzed on the method of combining morphology and cytology identification.The results showed that:there were no endosperm in the hybrid ovary,sometimes one dissociative globular young embryo could be found in few ovaries,which maybe grew into wheat plants,but their anthers were obsolete or pollens are yeld in the blooming period,which could be looked as a kind of morphology mark.In this study,the chromosome number of all hybrid progeny plants through wheat×maize were 2n=21,there was no homologous chromosome in whole genomes,and there was no maize chromosomes,also.On the basis of all above results and combining the character of chromosomal base number in Triticum L.plant is x=7,the hybrid progeny plants were identified as true wheat haploid(allotriploids),which was the most important cytology mark to identify haploid through wheat and maize cross.The importance of haploid identification in wheat × maize and the differences of previous relative results were also discussed in this paper.
【Fund】: 国家科技支撑计划(2006BAD01A02);; 农业科技跨越计划(2005跨07);; 948重大国际合作项目(20062G2b)资助
【CateGory Index】: S512.1
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