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《Journal of Nuclear Agricultural Sciences》 2008-05
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DIVERSITY ANALYSIS OF Brassica napus BY EST-SSR AND RAPD MARKERS

DONG Yuan-yuan YU Mi-na LI Xiao-bai XU Pan-feng CUI Hai-rui ZHANG Ming-long(Institute of Nuclear and Agricultural Sciences/Nuclear Agricultural Sciences key laboratory of Agricultural Ministry,Zhejiang University,Hangzhou,Zhejiang 310029)  
Parents matching is the key point in the strong portfolio for heterosis application.Heterosis strength is distance decided by genetic distance.35 varieties of Brassica napus,including 4 sterile lines,3 maintainer lines,10 restorer lines and 11 conventional varieties,were analyzed by EST-SSR and RAPD markers for the genetic diversity and heterosis.399 alleles were detected in the 35 varieties,of which 130 alleles accounting for 32.57% showed polymorphic.The range of the 35 varieties'genetic distance was between 0.0280~0.2801 computed by NTSYS(software).At the level of genetic distance 0.1401,the varieties were divided into 6 groups.Ogu,Polima and Shan 2A CMS were clustered into the fifth groups.Xin1 CMS had further genetic distance.Compared to maintainer lines and sterile lines,restorer lines and conventional varieties,showed more genetic diversity.It was described their diversity of genetic basis and pedigree sources on the whole.
【Fund】: 浙江省重大科技专项(2006C12019)
【CateGory Index】: S565.4
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