Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Nuclear Agricultural Sciences》 2009-01
Add to Favorite Get Latest Update


WANG Xiu-zhi~1 ZHANG Hong-yu~2 WANG Xu-dong~2 WU Xian-jun~2(1.HLJ August First Land Reclamation University,Daqing,Heilongjiang 163319;2.Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130)  
Gus and nptⅡ genes were constructed separately with their respective vectors and co-transformed into mature seed-derived calli of japanica rice cultivars Eyi 105 and Zhonghua 12 with a strain dosage ratio of 1∶1 and 1∶3 respectively through Agrobacterium tumefaciens-mediated transformation.21 transgenic plants contained gus gene were gained from 43 regeneration plants.The results of PCR showed that co-transformation efficiency of these two genes were 47.37% and 60.00%,respectively.The results of Southern Blot and Western Blot analysis showed that Gus had been integrated into rice genome.Genetic analysis confirmed Mendelian segregation was popular among T_1 plants in progeny.In this study,some key problems were discussed such as the relation of transformation rate and strain dos percent,which would be help to support the mutualism effect.
【CateGory Index】: S511
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 3 Hits
1 WANG You1,WU Yu1,WANG Yan1,LI Zhao-bo1,WANG Guang-xia2(1 Innovation Center of Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning,110161;2 Baita Subdistrict Office of Dongling District of Shenyang,Shenyang,Liaoning,110167,China);The Five Kinds of DNA Transformation Techniques[J];Rain Fed Crops;2010-03
2 JIN Yong-mei;MA Rui;YU Zhi-jing;LIN Xiu-feng;Agro-Biotechnology Research Institute, Jilin Academy of Agricultural Sciences;;Development of Transgenic Rice Plants Harboring Two Bt Genes by Agrobacterium-Mediated Co-Transformation[J];Journal of Jilin Agricultural Sciences;2014-05
3 WU Juan,XU Quan,HAN Jing,SUN Ting-ting,YANG Fang,MA Chang-jiang,WEI He-ping (Anqing Teachers’ College,Life Science School,Anqing 246011,China);Genetic transformation mediated by agrobacterium with fragrant gene in rice[J];Science and Technology of Food Industry;2011-07
Chinese Journal Full-text Database 7 Hits
1 Zhang Xiuchun~1 Peng Ming~1 Wu Kunxin~1 Guo Liqiong~(1,2) Lin Junyang Lin Junfang~(1,2) (1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;2 Department of Bioengineering,College of Food Sciences,South China Agricultural University,Guangzhou 510640;3 Putian City's Institute of Agricultural Sciences,Putian 351100);GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T-DNA BINARY VECTOR[J];Soybean Science;2006-04
2 TANG Li~(1,),LIU Qiao-Quan~(2,3,),DENG Xiao-Xiang~1,WU Xiao-Jin~1 and Samuel S M Sun~(2,*) (()~1Hunan Hybrid Rice Research Center,Changsha 410125,Hunan;()~2Department of Biology,Chinese University of Hong Kong,New Territory,Hong Kong;()~3College of Bioscience and Biotechnology,Yangzhou University,Yangzhou 225009,Jiangsu,China);LRP Transgenic Indica Rice Restorer Line without Resistance Selection Marker[J];Acta Agronomica Sinica;2006-08
3 ZHANG Hong Yu 1,2 ,WU Xian Jun 1,① ,TANG Ke Xuan 2,WANG Xu Dong 1, SUN Xiao Fen 2,ZHOU Kai Da 1 (1 Rice Research Institute of Sichuan Agricultural University,Wenjiang 611130,China; 2 Institute of Genetics,Fudan University,Shanghai 200433,China)[WT5”HZ];A Primary Study of Transferring the Pinellia Tenata Agglutinin (pta) Gene into Rice and Expression[J];Acta Genetica Sinica;2003-11
4 LIU Zhi,YUAN Xiao-ling,ZHANG Tian-zhen (Department of Genetics & Breeding, Nanjing Agricultural University; Key Laboratory of Crop Germplasm & Breeding, Ministry of Agriculture, Nanjing 210095,China);Strategies to Develop Transgenic Crops Expressing Multiple Genes[J];Hereditas(Beijing);2001-02
5 CHEN Rui1,2, CHENG Zai-Quan2, HUANG Xing-Qi2, ZHANG Wei1, 2 1. College of Life Sciences, Yunnan University, Kunming 650091, China; 2. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;Progress in rice genes mapping and gene distribution in chromosomes[J];Hereditas;2007-04
6 ZHU Li1,#,FU Ya-ping1,#,LIU Wen-zhen1,HU Guo-cheng1,SI Hua-min1,TANG Ke-xuan2,SUN Zong-xiu1,*(1State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China;2Plant Biotechnology Research Center,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200030,China);Rapidly Obtaining the Marker-Free Transgenic Rice with Three Target Genes by Co-Transformation and Anther Culture[J];Chinese Journal of Rice Science;2007-05
7 YU Heng-xiu1, LIU Qiao-quan1, WANG Ling1, 2, ZHAO Zhi-peng1, XU Li1, Huang Ben-li3, GONG Zhi-yun1, TANG Shu-zhu1, GU Ming-hong1 (1 State Key Laboratory of Plant Functional Genomics of the Ministry of Education, Yangzhou University, Yangzhou 225009; 2 China National Rice Research Institute, Hangzhou 310006; 3 Department of Plant Protection, Agricultural College, Yangzhou University, Yangzhou 225009 );Breeding of Selectable Marker-Free Transgenic Rice Lines Containing AP1 Gene with Enhanced Disease Resistance[J];Scientia Agricultura Sinica;2005-12
Chinese Journal Full-text Database 10 Hits
1 LIU Xiang-ling~(1,2),WANG Yu-zhen~1,LUO Jing-lan~1(1.Center for Agricultural Resources Research,Institute of Genetic and Developmental Biology,Chinese Academy of Science,Shijazhuang 050021,China;2.Graduate University of Chinese Academy of science,Beijing 100039,China);Study on callus induction and regeneration from rice mature embryo scutum[J];Shandong Agricultural Sciences;2005-05
2 LI Hai-qing1,2,3,ZOU Min4,YAO Fang-yin1,2,LIU Wei1,2 (1.High-Tech Research Center,Shandong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement and Biotechnology,Shandong Province,Jinan 250100,China;2.Key Laboratory of Crop Genetic Improvement and Biotechnology,Huanghuaihai,Ministry of Agriculture,Jinan 250100,China;3.College of Life Science,Shandong Normal University,Jinan 250014,China;4.The First Middle School in Laiwu,Laiwu 271100,China);Advances in Agrobacterium-Mediated Co-Transformation Systems for Marker-Free Transgenic Rice[J];Shandong Agricultural Sciences;2011-07
3 Ni Bin(College of Life Science Nanjing Agricultural University Nanjing Jiangsu 210095);Current Research Progress of Plant Marker free Transgenic Technology[J];Anhui Agricultural Science Bulletin;2007-16
4 Zheng Xueqin(College of life Sciences,Fujian Normal University,Fuzhou 350108,China);Application of Cre/loxp Recombination System in Excision of Selectable Marker Gene from Transgenic Plants[J];Anhui Agricultural Science Bulletin;2011-01
5 Wu Xiaoliang et al (College of Agronomy and Life Sciences Southwest Agricultural University, Beibei, Chongqing 400716);Advances of the Studies on Engineering in Rape Breeding[J];Journal of Anhui Agricultural Sciences;2004-01
6 LI Feng-ling et al (Rice Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130);Plant Lectin and its Application in the Genetic Engineering of Plant Resistance to Insects[J];Journal of Anhui Agricultural Sciences;2006-03
7 HOU Chun-xia et al (College of Forestry and Horticulture,Sichuan Agricultural University,Yaan,Sichuan 625014);Research of Antibiotics Species and Concentration on Loquat Anther Embryos Transformation by Agrobacterium tumefaciens[J];Journal of Anhui Agricultural Sciences;2007-16
8 ZHANG Jie et al(The College of Life Science,Agriculture University of Hebei,Baoding,Hebei 071001);Progress in Marker-free Transgenic Plants[J];Journal of Anhui Agricultural Sciences;2009-12
9 WANG Lan et al (Key Laboratory of Plant Molecular Breeding of Guangdong Province,College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642);Progress in Genetic Transformation Mediated by Agrobacterium tumefacien in Rice[J];Journal of Anhui Agricultural Sciences;2009-30
10 CHEN Xiu-qing(Chemical Engineering Department,Yangzhou Polytechnic Institute,Yangzhou,Jiangsu 225127);Research Progress of Hairy Roots Induced by Agrobacterium rhizogenes[J];Journal of Anhui Agricultural Sciences;2011-16
China Proceedings of conference Full-text Database 3 Hits
1 LI Lan-yu~1 CHEN Wei~2 YANG Qing-hui~1 LUO Yan-qing~2 DONG Yun-song~2 LI Gen-ze~2 WANG Jing-qiao~(2*) (1.College of Agronomy and Biotechnology,Yunnan Agrcultural University,Kunming,650201 The Key Laboratory of Yunnan Agricultural Biotechnology,Economic Crop Research Institute,Yunnan Agricultural Science Academy,Kunming 650223,China);Research on RNA interference and marker-free transformation of Fad 2 gene in brassica napus[A];[C];2007
2 ZHUO,Chunliu,CAI Jiongliang,FANG Junyang,ZHOU Ming,GUO Zhenfei (Biotechnology Laboratory for Turfgrass and Forages,Department of Plant Science,College of Life Sciences,South China Agricultural University,Guangzhou 510642:China.);Early light induced protein(ELIP) gene from Medicago falcata confers resistance to abiotic stresses[A];[C];2010
3 Yang Lichen,Yang Xiaoguang Institute of Nutrition and Food Safety,Chinese Center for Disease Control and Prevention,Beijing 100050,China;Progress on biosafety assessment of marker genes in genetically modified foods[A];[C];2003
Chinese Journal Full-text Database 10 Hits
1 Ma Guilian Zhang Qin (Agricultural Scientific and Technical Information Research Institute,SAAS,Shanghai 201106,China);Application of Biotechnology of the Agriculture in the World and Expect in China[J];Anhui Agricultural Science Bulletin;2007-19
2 Chen Xia Luo Shiqiao Duan Cuifang Zeng Rizhong(Rubber Research Institute /Ministry of Agriculture Key Lab for Tropical Crops Physiology,CATAS,Danzhou,Hainan,571737,P.R.China);Research Advance on the Transcription Factors in Higher Plants[J];Anhui Agricultural Science Bulletin;2008-09
3 Zhang Zhili (Hainan Academy of Agricultural Sciences Haikou city,571000);Research Progress on Plant Sulfite Oxidase and Gene[J];Anhui Agricultural Science Bulletin;2008-11
4 LI Ji-ping1,XU Chun-bo1,WANG Yong2,LI Xing-you1,ZHAO Haixia1(1 Grassland Research Institute,Chinese Academy of Agricultural Sciences,Huhhot,Inner Mongolia 010010,China;2 College of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018,China);Advences in Rosearch of transgenic technology in Alfalfa[J];Anhui Agricultural Science Bulletin;2008-15
5 Gao Junshan et al (Anhui Agricultural University,Hefei 230036);Summary of the Techniques and Methods of Plant Gene Transformafion[J];Journal of Anhui Agricultural Sciences;2003-05
6 Chen Kai (Department of Agriculture,Hebei Institute of Engineering,Handan,Hebei 057150);Mechanism of Material Browing in Plant Tissue Culture[J];Journal of Anhui Agricultural Sciences;2004-05
7 CUI Dong-ya et al(Department of Life Science,Yuncheng University,Yuncheng,Shanxi 044000);Research Progress of Spread and Prevention Mechanism of Plant Virus Disease[J];Journal of Anhui Agricultural Sciences;2007-08
8 LIU Ming et al(Department of Bioscience and Biotechnology,Dalian University of Technology,Dalian,Liaoning 116024);Analysis of the Factor Impacting the Gene Transferring with the Pollen-tube Pathway Method[J];Journal of Anhui Agricultural Sciences;2007-12
9 SUN Wei et al (School of Life Science,Capital Normal University,Beijing 100097);Progresses in Maize Genetic Transformation Techniques[J];Journal of Anhui Agricultural Sciences;2007-25
10 GUO Xiao-li et al (Department of Life Science of Hengshui College,Hengshui,Hebei 053000);Study on the Culture of Callus from Mature Embryos of Different Wheat Varieties[J];Journal of Anhui Agricultural Sciences;2008-01
【Secondary References】
Chinese Journal Full-text Database 4 Hits
1 XIA Si-lei(Wuxi Fisheries College of Nanjing Agricultural University,Wuxi,Jiangsu 214081);Construction Methods of Transgenic Fish[J];Journal of Anhui Agricultural Sciences;2011-14
2 WEI Jun-jie et al(Baoding University,Baoding,Hebei 071001);Study of Herbicide-resistant GM Maize[J];Journal of Anhui Agricultural Sciences;2012-14
3 GONG Hong-wei;Baoji Vocational Technology College;;The Harm and Cause of Transgenic Product[J];Ningxia Journal of Agriculture and Forestry Science and Technology;2013-08
4 LI Bi-xia;Zhuhai Entry-Exit Inspection and Quarantine Bureau;;Effects of Transgenic Technology on Food Security[J];Journal of Agricultural Catastrophology;2013-06
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WU Juan~(1,2)(1. Department of Life Science,Anqing Teachers’ College , Anqing 246011, China;2. Bioscience and Biotechnology College, Yangzhou University, Yangzhou 225009, China);Initial Mapping of Fragrance Gene in Rice[J];Journal of Anhui Technical Teachers College;2005-03
2 Zhang Xiuchun~1 Gou Liqiong~(1,2 *) Wu Kunxin~1 Lin Junyang~3 Lin Junfang~(1,2)(1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of TropicalAgricultural Sciences.Haikou 571101;2.Department of Bioengineering,College of FoodSciences,South China Agricultural University.Guangzhou 510640;3.Putian City'sInstitute of Agricultural Sciences.Putian,351100);AGROBACTERIUM-MEDIATED TRANSFORATION OF SOYBEAN WITH THE EXPRESSION VECTOR CARRYING TWO SEPARATE T-DNAs[J];Soybean Science;2005-04
3 Zhang Xiuchun~1 Peng Ming~1 Wu Kunxin~1 Guo Liqiong~(1,2) Lin Junyang Lin Junfang~(1,2) (1.State Key Laboratory of Tropical Crop Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101;2 Department of Bioengineering,College of Food Sciences,South China Agricultural University,Guangzhou 510640;3 Putian City's Institute of Agricultural Sciences,Putian 351100);GENERATING MARKER-FREE TRANSGENIC SOYBEAN PLANTS BY AGROBACTERIUM-MEDIATED TRANSFORMATION WITH DOUBLE T-DNA BINARY VECTOR[J];Soybean Science;2006-04
4 Zheng Jia-tuan,Wang Wu-qi, Zhang Jian-fu,Zhang Shui-jin,Zhao Ming-fu,Xiao Chen-he ,Tu Shi-hang (The institute of Rice and Wheat, Fujian Agricultural Academy of Sciences, Fuzhou, Fujian province,350019);The technology of integration of high blight-resistance gene Xa-23 of varieties to the super-hybridization rice[J];Fujian Science and Technology of Rice and Wheat;2004-04
5 CHEN Zhi-wei, GUAN Hua-zhong,WU Wei-ren, ZHOU Yuan-chang, HAN Qing-dian (College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China);The screening of molecular markers closely linked to rice blast resistant gene Pi-1 and their application[J];;2005-01
6 Postgraduate: Mr Zhu W Y Superviser:Dr & Prof Zhang G Q South China Agricultural University, Guangzhou, 510642;Mapping of the Specific Compatibility Gene S-e using Molecular Markers in Rice(Oryza sativa L.)[J];Molecular Plant Breeding;2003-04
7 Wu Changjun Jiang Gonghao Li Xin Liu Tao Xu Caiguo He Yuqing*National Key Laboratory of Crop Genetic Improvement and National Center of Crop Molecular Breeding, Huazhong Agricultural University, Wuhan, 430070;Dynamic Detection and Analysis of QTL for Resistance to the Brown Planthopper, Using a Doubled-haploid Rice Population[J];Molecular Plant Breeding;2005-04
8 Li Rongbai1,5,6* Li Lishu1,6 Wei Sumei2 Wei Yanping3 Chen Yingzhi1,5,6 Bai Delang4 Yang Lang1 Huang Fengkuan2 Lu Weili1 Zhang Xiangjun1 Li Xiaoyong5 Yang Xinqing5 Wei Yuanwen11 Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 2 Plant Protection Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 3 Agro-information Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 4 China National Hybrid Rice Research Center, Changsha, 410125; 5 Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007; 6 Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Gusngxi University, Nanning, 530005;The Evaluation and Utilization of New Genes for Brown Planthopper Resistance in Common Wild Rice (Oryza rufipogon Griff.)[J];Molecular Plant Breeding;2006-03
9 XIAO Han-xiang, HUANG Bing-chao,ZHANG Yang(Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China);Application of PSM maker linked to gene Gm6 in rice resistant breeding[J];Guangdong Agricultural Science;2005-03
10 ZHU Li-li , ZHU Cai-lei,WENG Qing-mei, HUANG Zhen, HE Guang-cun(Key Laboratory of Ministry of Education for Plant Development Biology,Life Sciences College of Wuhan University, Wuhan 430072,China);Research progress on brown planthopper resistance genes in rice[J];Hubei Agricultural Sciences;2004-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved