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《Journal of Nuclear Agricultural Sciences》 2009-03
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THE GENETIC TRANSFORMATION OF STRAWBERRY WITH WINTER FLOUNDER ANTIFREEZE PROTEIN GENE

SUN Rui-fen~1(LI Tian-ran)~2 AN Yu-lin~1 LI Kun~3 SHI Hui-qin~3 NIU Su-qing~1 ZHAO Jing-hui~4(1.Biotechnology Research Center,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Huhhot,Inner Mongolia 010031;2.College of Life Science,Inner Mongolia University,Huhhot,Inner Mongolia 010021;3.Institute of Horticulture,Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Huhhot,Inner Mongolia 010010;4.Vegetable Technology Extend Station of Huhhot,Huhhot,Inner Mongolia 010070)  
Antifreeze protein(AFP) of Pseudopleuronectes americanus gene encoding pre,pro and mature was introduced into strawberry(Fragaria × ananassa cv. Xingdu 2 and Allstar) by mediated Agrobacterium gene transfer of a binary vector,and the axenic leaf discs and lower part of petioles of strawberry were used as explants.The results showed that the foreign AFP gene encoding pre,pro and mature was integrated independently into the genome of six transgenic strawberry plants by PCR and PCR-Southern bloting analysis of seven Kmamycin-resistant transformed plants.Three regenerated Xingdu 2 lines were positive for pre,pro and mature,and their transformation efficiency was 1.56%,1.49%and 1.67%respectively.Three regenerated Allstar lines were positive for mature and its transformation efficiency was 4.05%.
【Fund】: 内蒙古自治区科技重点攻关项目(NK20030082)
【CateGory Index】: S668.4
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