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《Journal of Nuclear Agricultural Sciences》 2010-02
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GENETIC DIVERSITY OF SOYBEAN GERMPLASM BY RAPD MARKER

WANG Min YANG Wan-ming LI Run-zhi DU Wei-jun(College of Agriculture,Shanxi Agricultural University,Taigu,Shanxi 030801)  
In order to study and further use wild soybean germplasm,the genetic diversity of 40 soybean accessions including cultivars,landraces and wild soybeans were analyzed at DNA molecular level by random amplified polymorphic DNA(RAPD).Out of 50 RAPD primers,38 primers were selected to generate polymorphic DNA fragment.Total 407 Bands were identified,309 of which were polymorphic.The ratio of polymorphism was 75.92%,with 2 ～ 14 polymorphic bands per primer and averaged 8.1.Ratio of polymorphic bands in wild soybeans(94.35% ) was higher than those of cultivars(87.47% ) and landraces(83.54% ).Shannon-Weaver indices ranged from 0.5865 to 4.2133 and averaged 2.3377.The average Shannon-Weaver index in wild soybeans(2.2336) was higher than those in cultivars(1.7331) and landraces(1.6198).Genetic similarity ranged from 0.44 to 0.92,with a mean of 0.75.Genetic similarity in wild soybeans(0.6498) was less than those in landraces(0.7015) and cultivars(0.7177).Genetic similarity was 0.6599 between cultivars and landraces,0.6487 between cultivars and wild soybeans and 0.6045 between landraces and wild soybeans,respectively.The UPGMA result showed that 40 materials could be divided into six groups.Cultivars and landraces were divided into 2 different groups respectively and wild soybeans were divided into 4 different groups.It indicated that wild soybeans had broad genetic background and great variation,which could provide excellent germplasm for soybean practical breeding.The number of specific bands in wild soybeans was more than the total of them in cultivars and landraces.It also showed that cultivars and wild soybeans had significant difference at the DNA level.
【Key Words】： soybean cultivar landrace wild soybean RAPD genetic diversity
【Fund】： 国家自然科学基金项目(30471094和30100114);; 山西省科技攻关计划项目(051017);; 山西省高等学校青年学术带头人支持计划项目(200425)
【CateGory Index】： S565.1
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