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《Journal of Nuclear Agricultural Sciences》 2010-03
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SOMATIC EMBRYOID GENESIS INDUCTION AND PLANT REGENERATION in vitro IN Glycyrrhiza uralensis Fisch

CAO Jun-mai LI Qiang WANG Chong (College of Biologcial Science&Engineering,The North University for Ethnics,Yinchuan,Ningxia 750021)  
Using the orthogonal experiment design of L9 (34 ),the effects of explants uralensis,hormone types and combinations on inducing embryoids,embryoids induced seedling cultivation and cultivation techniques were researched. The results showed that the best explant was the shoot of Glycyrrhiza uralensis cultured by embryoid induction and the optimal induction medium was MS + glycine 0. 3g /L + sucrose 30g /L + agar 5g /L + 2,4-D 0. 2mg /L. The obvious promotion of 2,4-D to embryoid induction was observed. The shoot was the best explant which could induce the seedling from the embryoid and the plantlet regeneration rate was 66. 67% with the medium of MS. The exogenous hormone inhibited the induction of plantlet from embryoid.
【Fund】: 宁夏自然科学基金(NZ0953)
【CateGory Index】: S567.71
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