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《Journal of Nuclear Agricultural Sciences》 2010-04
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SHI Hai-chun QIAO Xiao KE Yong-pei FU Ti-hua YU Xue-jie QIN Jia-you(Agronomy College,Sichuan Agricultural University,Ya’an,Sichuan 625014)  
To provide the foundation of distant crossing breeding in cell and molecular level,karyotype and SSR analysis between the distant inbred lines from Zea mexicana schard × K169 and their recurrent parent K169 were done.The results indicated that: the chromosome number in each somatic cell was 2n = 20,but there was obvious difference in chromosome structure.The inbred-line K169 and 1191 were found to carry a satellite on the short arm of 6th chromosome,1183 and 1189 on the short arm of 8th chromosome,1193 and 1195 weren’t found to carry a satellite.The chromosome relative length ratio(Lt /St) of 1183,1193 and 1195 was different from inbred-line K169.The karyotypes of 1183 and 1195 werent consistent to inbred-line K169.The results of SSR analysis showed that the 5 distant inbred Lines and their recurrent parent K169 were conducted with 27 SSR markers having polymorphism from a total of 96 pairs of SSR primers.The average value of polymorphism information content(PIC) was 0.724,and the average value of genetic diversity indexes(H’) was 1.400,the average value of genetic similarity coefficient of SSR marker pattern among these materials was 0.5791.According to the analysis results,genetic difference was existed between these distant inbred lines and their recurrent parent K169,and these distant inbred lines were new genetic resources.
【Fund】: 四川省教育厅青年基金项目(07ZB062);; 四川省教育厅重点专项(08ZZ002);; 四川省应用基础研究项目(2008JY0101);; 四川省“十一五”玉米育种攻关项目(2006-3-16)
【CateGory Index】: S513
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