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《Journal of Nuclear Agricultural Sciences》 2011-04
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ANTIOXIDANT ACTIVITIES in vitro AND MAIN EFFICACY COMPONENTS IN SUMMER MULBERRY LEAVES

PAN Jian-yong WANG Zhi-ping DANG Jiang-bo XIE Yan-guang DONG Dan-dan SHAO bin WANG Jing-mei LAN Jin-jin CHEN Zi-yuan(Institute of Nuclear-Agricultural Sciences of ZhejiangUniversity,Key Laboratory of Chinese Ministry of Agriculture for Nuclear-Agricultural Science,Hangzhou,Zhejiang 310029)  
Scavenging activities of ·OH,O-2· and 1O2 of summer mulberry leaves and the winter mulberry leaves were determined by evaluation technology of scavengingfree radicals in vitro.The results showed that the ·OH and O-2 scavenging activities of summer mulberry leaves were similar to that of winter mulberry leaves,and the capacities to 1O2scavenging activity of summer mulberry leaves were even 61% higher than that of the winter mulberry leaves.It indicated that the summer mulberry leaf is also a kind of ideal natural resources for medicine and health food.Furthermore,using 3 different extracting methods of dried leaves with hot water(Process 1),extracting from fresh leaves with hot water(Process 2) and extracting from fresh leaves with warm water(Process 3),9 extracts were obtained from the upper,middle and lower parts of summer mulberry leaves,respectively.The antioxidant activities and main components contents(polyphenols,flavonoids,polysaccharides and proteins) of the extracts were measured,and the correlations between antioxidant activities and main components were analyzed.The results were as follows:(1) The correlation between the antioxidant capacities and polyphenols and flavonoids contents were highly significant,and the contents of polysaccharides were only significantly correlated with the capacity for scavenging1O2,while the proteins were almost no scavenging radicals capacity.It indicated that the flavonoids and polyphenols in the summer mulberry leaves played the main role of antioxidation just as in winter mulberry leaves.(2) The effects of leaf position and extraction method on the antioxidant activities and contents of main components in summer mulberry leaves needed to be considered.(3) As for extracting the bioactive substances from the summer mulberry leaves,it's better to choose the middle and upper leaves and the Process 1.In addition,if the material was fresh,it's better to choose the middle leaves and the Process 2.And the upper or lower leaves might also be utilized in some cases.
【Fund】: 国家公益性行业(农业)科研专项(200803034);; 国家自然科学基金项目(30771669);; 浙江省院士基金(J20080388)
【CateGory Index】: S888
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