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《Journal of Nuclear Agricultural Sciences》 2015-03
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Identification and Analysis of a Rice Lignin Monomer Synthesis Deficient Mutant

CHEN Lei;LI Qiuyuan;YE Shenghai;JIN Qingsheng;ZHANG Xiaoming;Institute of Crops and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Science;Nanjing Institute for the Comprehensive Utilization of Wild Plants;College of Life and Environment Sciences,Shanghai Normal University;  
A brittle stem mutant ‘Zhejing 88GH'was obtained from japonica rice variety ‘Zhejing 88 'by irradiated mutation with60 Co gamma rays. M( a) ule reagent was used to measure the composition of lignin in ‘Zhejing 88 GH'.Lignin monomer composition in cortex and vascular bundle area in the mutant is different from that in the wild type‘Zhejing 88'. PCR and gene sequencing for the candidate genes within the target regions of 90 kb on Chromosome 2showed that there was a mutation in the 191 st base in the first exon( C to G) of LOC_Os02g09490. The gene family spectrum and the three-dimensional structure of LOC_Os02g09490 protein were also analyzed. qRT-PCR result showed that this gene was constitutively expressed in roots,stems and leaves in ‘Zhejing'88 while has low expression level or even no expression in ‘Zhejing88 GH'. Furthermore,with subcellular localizations of two vectors using Agrobacteriummediated transformation,LOC_Os02g09490 was first localized in both nucleus and cell membrane.
【Fund】: 国家自然科学基金(31101428);; 浙江省9410计划(2012C12901-2);; 浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地;; 浙江省农业科学院科技创新能力提升工程项目
【CateGory Index】: S511
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Chinese Journal Full-text Database 10 Hits
1 FU Yue et al (College of Bio & Food Tech, Dalian Institute of Light Industry, Dalian,Liaoning 116034);Cloning and Identification of Lignin Biosynthesis F5H Gene[J];Journal of Anhui Agricultural Sciences;2006-08
2 HUANG Chun-qiong et al (Institute of Tropical Crop Genetic Resource,China Academy of Tropical Agricultural Sciences,Danzhou,Hainan 571737);Lignin Biosynthesis Regulated by Antisense CCoAOMT Gene in Tobacco[J];Journal of Anhui Agricultural Sciences;2008-19
3 LI Wei,XIONG Jin,CHEN Xiaoyang(College of Biological Sciences and Biotechnology,Beijing Forest University,Beijing 100083,China);Advances in the research of physiological significances and genetic regulation of lignin metabolism[J];Acta Botanica Boreali-occidentalia Sinica;2003-04
4 XU Shixiao, ZHAO Xinquan, SUN Ping,ZHAO Tongbiao,ZHAO Wei(Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining 810001,China);A study on acid detergent lignin content and digestibility of 5 species herbage in Tibetan Plateau[J];Acta Botanica Boreali-occidentalia Sinica;2003-09
5 NIE Hui-zhong,XUE Yong-chang (School of Biology & Food Technology,Dalian Polytechnic University,Key Laboratory of Ferment Engineering,Dalian,Liaoning 116034,China);Evolution Analysis of c3h cDNA Fragment from Poplar[J];Acta Botanica Boreali-Occidentalia Sinica;2008-05
6 CHEN Yong-zhong 1,TAN Xiao-feng 2, David Clapham 3 (1. Hunan Academy of Forestry, Changsha 410004,China; 2. Central South Forestry University,Zhuzhou 412006,China; 3. Swedish University of Agricltural Sciences);Lignin Biosynthesis and Genetic Regulation[J];Acta Agriculturae Universitis Jiangxiensis;2003-04
7 Xue Yongchang(College of Bio. and Food Tech.,Dalian Institute of Light Industry Dalian116034) Li Jinhua Lu Mengzhu Zhang Qiwen(The Research Institute of Forestry, Chinese Academy of Forestry Beijing100091);THE LIGNIN SUBUNITS BIOSYNTHESIS PATHWAY AND ITS REWRITING[J];Scientia Silvae Sinicae;2003-06
8 LI Qiu-yuan1,2,3 YE Sheng-hai2 ZHANG Ying-ying3 MA Xiao-jing1 MEI Jie1 JIN Qing-sheng2 HE Zu-hua3 DONG Yan-jun1 ZHANG Xiao-ming2(1.Laboratory of Plant Genetics and Functional Genes,College of Life and Environment Sciences,Shanghai Normal University,Shanghai 200234; 2.Institute of Crop and Nuclear Technology Utilization,Zhejiang Academy of Agricultural Science,Hangzhou,Zhejiang 310021; 3.Insititute of Plant Physiology and Ecology,Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences,Shanghai 200032);MOLECULAR MAPPING OF A GOLD HULL AND INTERNODE GENE IN Oryza sativa L.[J];Journal of Nuclear Agricultural Sciences;2012-07
9 XIANG Dian-jun;MAN Li-li;ZHANG Di;WANG Li-na;YIN Kui-de;SONG Qun-yan;XU Zheng-jin;Heilongjiang Agricultural Economy Professional College;Rice Institution of Shenyang Agricultural University/Key Laboratory of Crop Physiology,Ecology,Genetics and Breeding,Ministry of Agriculture/Key Laboratory of Northern Japonica Rice Breeding of Liaoning;Daqing Branches of Heilongjiang Academy of Agricultural Sciences;School of Life Science and Biotechnology,Heilongjiang Bayi Agricultural University;;Expression of LsICE1 Gene and Its Regulation Function of Cold Responsive Signalling Pathway in Transgenic Rice[J];Journal of Nuclear Agricultural Sciences;2013-10
10 WANG Zhong;ZHANG Yue-qiang;FAN Zhe-ru;ZHAO Qi;LI Jian-feng;ZHANG Hong-zhi;WANG Zi-xia;Haireguli·Abulizi;Institute of Nuclear and Biological Technologies,Xinjiang Academy of Agricultural Sciences;;Cloning of Zmpepc Gene from Maize and Transformation into Spring Wheat[J];Journal of Nuclear Agricultural Sciences;2013-11
Chinese Journal Full-text Database 10 Hits
1 LI Ji-ping1,XU Chun-bo1,WANG Yong2,LI Xing-you1,ZHAO Haixia1(1 Grassland Research Institute,Chinese Academy of Agricultural Sciences,Huhhot,Inner Mongolia 010010,China;2 College of Ecology and Environment,Inner Mongolia Agricultural University,Huhhot,Inner Mongolia 010018,China);Advences in Rosearch of transgenic technology in Alfalfa[J];Anhui Agricultural Science Bulletin;2008-15
2 FU Yue et al (College of Bio & Food Tech, Dalian Institute of Light Industry, Dalian,Liaoning 116034);Cloning and Identification of Lignin Biosynthesis F5H Gene[J];Journal of Anhui Agricultural Sciences;2006-08
3 FU Yue et al (College of Bio.& Food Tech.,Dalian Institute of Light Industry,Dalian,Liaoning 116034);Research Advance on Lignin Biosynthesis and Genetic Regulation[J];Journal of Anhui Agricultural Sciences;2006-09
4 JIN Shun-yu et al (SFA Key Open Laboratory on Bamboo and Rattan Science and Technology,International Center of Bamboo and Rattan,Beijing 100102);Research Progress of Lignin Biosynthesis with Genetic Engineering Technology and Its Application Prospect in Bamboo Improvement[J];Journal of Anhui Agricultural Sciences;2008-20
5 XIE Ling-li et al (College of Life Science,Yangtze University,Jingzhou,Hubei 434025);Study on Preparation of Concentrated Protein from Double-low Rape Seed[J];Journal of Anhui Agricultural Sciences;2009-01
6 WANG Lin et al (College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083);Construction of Plant Expression Vector with Bivalent Genes XERICO and Tyr-rich HRGP[J];Journal of Anhui Agricultural Sciences;2009-29
7 Zhang Zhiyi; Lin Shanzhi; Zhang Deqiang; Zhang Qian. College of Biological Science and Biotechnology,Beijing For.Univ., 100083, P. R. China.;Applications of modern molecular biotechnology to genetic improvement in forest trees.[J];Journal of Beijing Forestry University;2002-Z1
8 CAO Ai-juan1a,WANG You-nian2,3,GE Shui-lian4,YANG Ai-zhen1b,XIN Yue-jun5,HUA Bao-guang1b(1.a.Department of Plant Science and Technology; b.Department of Biotechnology,Beijing University of Agriculture,Beijing 102206,China; 2. Key Laboratory of New Technology of Agricultural Application,Beijing 102206,China;3. Key Laboratory of Urban Agriculture (North),Ministry of Agriculture,Beijing 102206,China;4. Department of Biological Science,Handan College,Hebei 056006,China;5.Management Center of Agricultural Production in Beijing Southern Suburb,Beijing 100076,China);Changes of Enzyme Activity during the Depositive Process of Lignin in Peach Endocarp[J];Journal of Beijing University of Agriculture;2009-01
9 CHEN Xie(College of Food Science, Southwest University, Beibei CHongqing 400716, China);Research Present Condition of Lignin Change and Biosynthesis Control of Bamboo Shoots During Preservation[J];Storage & Process;2006-03
10 XIE Xin-ming,ZHAO Yan-hui,HUO Song,CHEN Hui (College of Agriculture,South China Agricultural University,Guangzhou,Guangdong Province 510642,China);Dynamic Changes of Enzyme Activities Related to Lignin Biosynthesis for Elephantgrass Cultivars[J];Acta Agrestia Sinica;2010-04
China Proceedings of conference Full-text Database 1 Hits
1 Wang Kang~1,Dong Jie~2,Zhou He~(1*) (1.Institute of Grassland Science,China Agriculture University,Beijing 100193,China 2.Institute of animal science,CAAS,Beijing 100193,China);Effects of Transgenic Technology on the Molecular Breeding for Turfgrass Plants[A];[C];2010
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHAO Yan et al(Biotechnology Research Center,Kunming University of Science and Technology,Kunming,Yunnan 650224);Molecular Catalytic Mechanism of Major Enzyme-Phosphoenolpyruvate Carboxylase in C_4 Photosynthesis[J];Journal of Anhui Agricultural Sciences;2006-23
2 LIU Jin xiang 1, HU Zi zhi 2, LIANG Xiu 3, SU Wen juan 3 (1.Institute of Grassland Science, Northeast Normal University, Changchun 130024, China; 2.Pratacultural Science College, Gansu Agricultural University, Lanzhou 730070, China; 3.Huangcheng;A serial study of grazing ecology and digestibility and metabolism of sheep on alpine pasture Ⅲ The analysis of seasonal dynamics and the restrictive factors of the diet digestibility by grazing sheep[J];Pratacultural Science;2001-02
3 ZHAO Yi-ling1,GUO Zhao1,SUN Hui2,XUE Yong-chang1(1.School of Biological & Foodstuff Engineering,Dalian Institute of Light Industry,Dalian 116034,China; 2.School of Chemistry Engineering & Material,Dalian Institute of Light Industry,Dalian 116034,China);Optimization of PCR condition of lignin biosynthesis CCR gene[J];Journal of Dalian Institute of Light Industry;2007-01
4 XU Shi xiao,ZHAO Xin quan,SUN Ping,ZHAO Wei,ZHAO Tong biao (Northwest Plateau Institute of Biology,Chinese Academy of Sciences,Xining 810001,China);Study on simulated vitro digestibility of 2 species sedge family herbage grown at different altitudes in Tibetan Plateau[J];Acta Botanica Boreali-occidentalia Sinica;2002-05
5 GENG Sa,XU Cunshuan,LI Yuchang of Henan Province,Xinxiang,Henan 453002,China);Advance in biosynthesis of lignin and its regulation[J];Acta Botanica Boreali-occidentalia Sinica;2003-01
6 CHEN Jie1,DENG Jing1,CHEN Jian-rong2,XU Lei1,ZHANG Xue-wen1*(1 College of Bioscience and Biotechnology of Hunan Agricultural University,Changsha,Hunan 410128,China;2 Institute of Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha,Hunan 410205,China);Transformation of Model Tobacco Variety WS38 with Antisense CCoAOMT cDNA of Boehmeria nivea[J];Acta Botanica Boreali-Occidentalia Sinica;2007-08
7 Cong Bin; Yang Maocheng; Su Bo; Zhang Pifang(Fudan University, Shunghui First Pulmonary Hospital);Changes in enzymatic activity during the period of cell differentiation of wheat (Triticum aestivum) root tip[J];JOURNAL OF FUDAN UNIVERSITY;1997-05
8 Lin Yuanzhen1,2 Zhang Zhiyi2 Liu Chunxin1 Guo Hai3 Zhu Baoqing2 Chen Xiaoyang1 1 College of Forestry, South China Agricultural University, Guangzhou, 510642; 2 Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, 100083; 3 Plant Materials for Soil and Water Conservation, Ministry of Water Resources, Beijing, 100038;in silico Cloning and Analyzing of PsICE1 from Populus suaveolens,A Freezing-resistant Transcription Factor[J];Molecular Plant Breeding;2007-03
9 Huo Shiping ;Yan Qinjiou ;Sun Guangyin ;Su Minlun(Institute of Agroscience of Wanxian City, Wanxiah, Sichuan Province. 634000);Progress in Morphological and Physiological and Biochemical Indexes of Drought Resistance Identification of Maize[J];AGRICULTURAL RESEACH IN THE ARID AREAS;1995-03
10 YUAN Hong-xu1,LIU Wan-gou1,LIU Yue-lian2,GUO Jian-fu2,LI Yue-ren3(1 Life Science and Technology School,Zhanjiang Normal University,Zhanjiang,Guangdong 524048,China;2 Institute of Agricultural Biotechnology,Guangdong Ocean University,Zhanjiang,Guangdong 524088,China;3 Central Laboratory,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China);Inheritance analysis on sheath blight resistance and agronomic traits of transgenic rice transformed with chitinsase-gene[J];Journal of Southern Agriculture;2012-02
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