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STUDY ON HUMAN G CSF EXPRESSION BY INJECTING PLASMID INTO COW MAMMARY

Wang Gui (Jilin Agricultural University, Changchun 130018) Lu Yifan\ Deng Jixian\ Tian Chai (Institute of Biotechnique, Academy of Military Medince, Beijing 100071)  
Expression plasmid with whey acid protein (WAP) gene 5′ as a control region and human G CSF gene as a target was constructed. This plasmid was injected into cow mammary by injector. The results showed that there were 732μg/ml human G CSF in cow milk.It is suggested that this method might be used as a new system to produce recombinant protein in future. \ \
【CateGory Index】: S852.2
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【Co-references】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Xue-min,WANG Jin-sheng (Key Lab. of Monitory and Management of Plant Diseases and Insects, Ministry of Agriculture of Nanjing Agric. Univ.,Nanjing 210095,China);A Modified Alkaline Lysis Method for the Preparation of Plasmid DNA[J];Journal of Anhui University(Natural Sciences);2000-04
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3 GUAN Xiao-bin et al(The College of Animal Science and Technology,Sichuan Agricultural University,Yaan,Sichuan 625014);Construction of the Mammary Gland-specific Expression Vector by Bovine αS1-casein Gene[J];Journal of Anhui Agricultural Sciences;2006-23
4 Sun Zhan sheng Chen Jin chun (College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China);Repression of rHSA degradation during fermentation with transgenic yeast pichia pastoris[J];Journal of Beijing University of Chemical Technology;2004-04
5 LI Yunfeng,Shao Guo,Su Yan,et al (Biology Department,Inner Mogolia University, Huhhot 010021, China);A Repid and Easy Way of Extracting and Purifying Plasmid DNA[J];JOURNAL OF BAOTOU MEDICAL COLLEGE;2000-02
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7 WANG Han-sen, PAN Hong, ZHANG Yu-zhi, BAO Xin-hua, ZHANG Guo-rong, WU Xi-ru △(Department of Pediatrics, Peking University First Hospital, Beijing 100034, China);Helper-virus-free herpes simplex virus-1 vector mediated LacZ gene expression in cultured cortical neurons[J];Journal of Peking University(Health Sciences);2005-02
8 ZHOU Xing1,2,ZHANG Ju-nong1 (1.College of Animal Science and Technology,Shihezi University,Shihezi 832003,China);Construction of Recombinant Retroviral Vector Containing Lumbrokinase Gene and its Expression in Cow Milk[J];Grass-Feeding Livestock;2007-01
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10 JIA Xiang Zhi 1, YUAN Han Ying 2, MA Wen Yu 1, LI Yuan 1, LI Yu Yang 2 1Department of Microbiology, Fourth Military Medical University, Xi'an 710033, China, 2Institute of Genetics, Fudan University, Shanghai 200433, China;Cloning and expression of human lysozyme gene in Pichia pastoris[J];Journal of The Fourth Military Medical University;2001-22
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 DING Yi 1) , WANG Feng 1) , LE Xiaoping 1) , MA Kaiyan 2) , ZHANG Qinxian 1) 1) Department of Histology & Embryology, College of Basic Medical Sciences ,Zhengzhou University, Zhengzhou 450052 2) Henan Key Laboratory of Molecular Medicine, Zhengzhou 450052;Transient expression of tissue-type plasminogen activator in mammary gland tissues of mice[J];Journal of Zhengzhou University(Science Medical);2004-03
2 ZHANG Quan1 ZHU Hong-fei2 YU Ke-xiang1 XUE Fang-ming1 SUN Huai-chang1(1 Collge of Veteriniary Medicine Yangzhou University,Yangzhou 225009,China)(2 Institute of Animal Science and Veterinary Medicine Chinese Academy of Agricultural Science,Beijing 100081,China);Modification of Alkaline Lysis Method for Preparation of Plasmid DNAs from Recombinant E.coli[J];China Biotechnology;2007-02
3 LI Guo-cai *, SUN Huai-chang, SUN Qiang, CHEN Gang, ZHANG Quan, SHI Wei-qing, LI Hou-da. *Collegy of Medical, Yangzhou University, Yangzhou 225009, China;Transgenic mice expressing human lysozyme gene in milk[J];Chinese Journal of Microbiology and Immunology;2004-02
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