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《(拉丁文)ACTA AGRICULTURAE NUCLEATAE SINICA》 1999-04
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STUDIES IN GETTING HYBRIDS PLANTS OF INTERSPECIFIC\=HYBRIDIZATION THROUGH OVULE CULTURE IN VITRO

Shi Xiangyu\ Gao Guoqiang\ Su Xuehe\ Zhu Doubei\ Chen Xiao (Institute for Application of Atomic Energy, Shandong Academy of Agriculture Sciences, Jinan\ 250100) Cui Ping\= (Shandong Education College, Jinan\ 250013)  
Ovule culture was made on Gossypium hirsutumG.arboreum, G.hirsutumG.barbadense, G.vc singal stem mutantG.barbadense, G.barbadenseG.arboreum. After 8590 days of culture embryos (including matured and unmatured ones) as well as seedling from matured embryo were obtained from these 4 crosses with a certain frequency. The results of experiment showed that the differences were significant between embryo frequency and germination percentage of embryos with ovule culture on different media and the culture with ovules from different crosses, and on the same medium the difference was great.\;The ovules from hybrids having developed for different days after pollination were cultured on BT,BT medium in the darkness. Embryo development with a high frequency was obtained with ovules from G.hirsutumG. barbadense and G.hirsutumG. arboreum 46 days after pollination.
【CateGory Index】: S562.035.3
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