EFFECT OF ANISODAMINE ON ARRHYTHMIAS IN CALCIUM PARADOX ISOLATED RAT HEARTS
Guo Dianling Luo Han Chen Guangwen Luo Zhengyao Department of Pathophysiology, Hunan Medical University
Drug effect of anisodamine in reducing the incidence of arrhythmias of the calcium paradox isolated rat hearts was observed. Fifty fwo male Wistar rat isolated hearts were randomly divided into: 1) blank control group (N=10) , 2) calcium paradox control group (N = 22), and 3) amisodamime calcium paradox group A (N=11), in which anisodamine (3. 28 ×10-4mol/L) , was added in to perfusate during the stable and calcium-depletion phase, and 4) anisodamine calcium paradox group B (N = 9), only the calcium-repletion phase used anisodamine. Epicardial ECG, LDH in coronary effluent and energy eispersive X-ray spectrum identified histocytochemical utrastructrual distribution of calcium were assayed. Incidence of arrhythmias, mitochondrial calcium deposition, and LDH released in the coronary effluent 1st mih of calcium-repletion phase all decreased significantly in the group A but not in the group B as compaired with the calcium paradox contorl group. Correlation between the arrhthmias and calcium influx that was inhibited by anisodamine when existedl.