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AN IMPROVED FLUOROMETRIC METHOD IN DETERMINATION OF PLASMA GLUTATHIONE

Tang Aiguo Yang XinanCentral Laboratory. The Second Affiliated Hospital. Hunan Medical University  
An improved fluorometric method for measurement of plasma glutathione is introduced. 10% trich loroacetic acid and pH 8.3 phosphate buffer solution were used instead of 25% HPO3 and pH 8.0 phosphate buffer solution respectively and the method might be performed under room temperature. The repetition tests and recovery tests of the method were favorable giving a sensitivity of 25ng /ml and a linear standard curve to at least 2500ng/ml. Plasma glutathione determined by this method in 39 healthy blood donators showed a result of 15.125± 3, 38μmol/L and no any sexual difference. The method is sensitve, accurate and less expensive.
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