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《Bulletin of Hunan Medical University》 2003-04
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Effect of Coptis Chinensis compound on the gene expression in transplanted tumor tissue in nasopharyngeal carcinoma cell line of CNE1 by cDNA microarray

WANG Guang-ping 1,2, TANG Fa-qing 2, ZHOU Jin-ping 3(1. Department of Hematology; 2. Central Laboratory, Xiangya Hospital,Central South University, Changsha 410008, China;3.Department of Pathology, Second Xiangya Hospital, Central South University, Changsha 410011, China)  
Objective To investigate the effect of Coptis Chinensis compound on the gene expression of transplanted tumor in nasopharyngeal carcinoma cell line of CNE1. Methods The cells were injected into Balb/C nude mice, and after the transplanted tumor was established , the nude mice with tumors were treated with Coptis chinensis compound. The nude mice were then killed, tumor tissues were isolated and the total RNA was extracted. Hybridization with a cDNA microarray was conducted using a probe of fluorescent-labeled cDNA mixture that was reversely transcribed from the RNA. The gene expression level was analyzed after scanning the fluorescent intensity by a laser scanner, and further confirmed by RT-PCR. Results After using Coptis Chinensis compound for 30 days, the size of transplanted tumors were remarkably reduced, and the inhibition rate of tumor growth was 29.5%. In the meantime, the expression of 147 genes in the tissue of implanted tumor treated with the compound altered:the expression of 102 genes was down-regulated, and that of 45 genes was up-regulated. Furthermore, the differential expression of 3 genes was verified by RT-PCR, MAD3 and H731 genes were up-regulated, and that of CHK1 was down-regulated. Conclusion Coptis Chinensis compound can affect the gene expression in the tissue of implanted tumor, which implies that the inhibition of Coptis Chinensis compound on the growth of the implanted tumor with CNE1 cells in nude mice might be associated with the control of gene expression.
【Fund】: 国家自然科学基金 (项目编号 30 0 70 945 )
【CateGory Index】: R739.63
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Chinese Journal Full-text Database 7 Hits
1 YU Yuan-yuan,WANG Bo-chu,PENG Liang,WANG Jing-bo,ZENG Chen(Key Laboratory for Biomechanics and Tissue Engineering Under the State Ministry of Education,College of Bioengineering,Chongqing University,Chongqing 400030,China);Advances in Pharmacological Studies of Coptis Chinesis[J];Journal of Chongqing University(Natural Science Edition);2006-02
2 WANG Hai-lun1,2,HU Bo1,2, SU Qi1(1.Cancer Research Institute,University of South China,Hengyang 421001,China;2.College of Nursing,Hunan Environment-Biological Polytechnic,Hengyang 421001,China);The Application of Gene Chip Technology in Research of Nasopharyngeal Carcinoma[J];Journal of Hunan Environment-Biological Polytechnic;2009-02
3 WANG Guang-ping, CHEN Fang-ping, FU Gan(Department of Hematology, Xiangya Hospital, Central South University, Changsha 410008, China);IκBα mRNA expression and its DNA sequence in nasopharyngeal carcinoma cell lines[J];Journal of Central South University(Medical Sciences);2007-05
4 Luo Biao Wei Qihou Liang Jin et al Department of Affiliated Hospital of Youjiang Madical College for Nationalities(Baise 533000);Study the effect of berberine on the telomerase activity of human nasopharyngeal carcinoma(NPC) CNE-2[J];Shaanxi Medical Journal;2007-10
5 YIN Hui,WANG Guang-ping, YI Hong, LENG Ru-yi, QI Zhen-hua, FU Gan, CHEN Fang-pingXiangya Hospital of Central South University, Changsha 410008, China.;Induction of apoptosis in CNE1 cells of nasopharyngeal carcinoma by recombinant mutant IκBα cDNA[J];Journal of Modern Oncology;2007-04
6 TANG Xiong-Zhi,YIN Ling.Department of Gynecology and Family Planning,People's Hospital of Guilin,Guilin 541002,Guangxi,China;The apoptosis of human cervical cancer Hela cell line induced by berberine[J];Maternal and Child Health Care of China;2010-30
7 CHEN Wan-tao.(Department of Oral and Maxillofacial Surgery,College of Stomatology,Ninth People's Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai Key Laboratory of Stomatology.Shanghai 200011,China);Investigation of molecular biology of oral maxillofacial and head & neck squamous cell carcinoma[J];China Journal of Oral and Maxillofacial Surgery;2008-03
Chinese Journal Full-text Database 10 Hits
1 Chen Guoliang, Ye Shoushan, Liu Jajun, et al AnHui Institute of medical sciences, Hefei 230061;Observation of antibacterial effect of JinDiLan tablet in infected mice body[J];Anhui Medical Journal;2002-05
2 WANG Shi-hong~(1,2),WEI Wei~(1),XU Du-juan~(1),XU Shu-ping~(1),CUI Ying~(2)(1.Institute of Clinical Pharmacology,Anhui Medical University;Anhui Key Laboratory of Antiinflammatory and Immunopharmacology;Anhui Provincial Key Laboratory of R&D of Chinese Medicine,Hefei 230032;2.He'nan College of TCM,Zhengzhou 450008);Proliferation inhibition of TGP on SMMC-7721 Cell in vitro[J];Anhui Medical and Pharmaceutical Journal;2006-01
3 YAN Rui-qi LI Jun-li HUANG Mei-ling HUANG Xiao-yan WANG Hui-yun FENG Qi-sheng (Cancer Institute, Sun Yat-sen University of Medical Sciences. Guangzhou, 510060);PROTECTIVE EFFECT OF GLYCYRRHIZIN ON DNA REPALR DURING ITS INHIBITION ON DEN-INDUCED HEPATOCARCINOGENESIS[J];Chinese Journal of Cancer;1995-04
4 Yang Huiling;Yu Qingsheng;Jian Zhihan; et al.(Department of Pathophysiogy, Sun Yat-Sen University of Medical sciences, Cuangzhou, 510080);Experimental study of inhibitory effect of naja naja actra venom on nasopharyngeal cancer and others[J];Chinese Journal of Cancer;1999-01
5 WANG Cheng-xing, LI Xiao-yan, CAO Ya, et al. (Cancer Research Institute of Hunan Medical University, Changsha 410078, P. R. China);Epstein-barr virus-encoded latent membrane protein 1 activates NF-kB via a mechanism involving TRAF2 in nasopharyngeal carcinoma[J];CHINESE JOURNAL OF CANCER;2000-06
6 CHEN Yang-chao,ZHANG Yue-fei,ZHOU Ke-yuan * Institute of Biochemistry and Molecular Biology,Guangdong Medical College, Zhanjiang 524023,P.R.China;Preliminary Studies of Apoptosis in Human Nasopharyngeal Carcinoma CNE -2Z Cells Induced by Arsenic Trio xide and Its Mechanism[J];Chinese Journal of Cancer;2001-10
7 SHAO Jian-yong1, ZENG Wei-fen2, ZENG Yi-xin1*1. Cancer Center, Zhongshan University, Guangzhou 510060, P.R.China.2. Department. of Pathology, Zhongshan University,Guangzhou 510060, P.R.China.;Molecular Genetic Progression on Nasopharyngeal Carcinoma[J];Chinese Journal of Cancer;2002-01
8 FENG Gong kan, XIE Bing fen*, LIU Zong chao, ZHU Xiao feng, YE Yan liCancer Institute,Cancer Center,Sun Yet sen University,Guangzhou 510060,P.R.China;Tea Polyphenol Inhibit on Growth of Human NasopharyngealCarcinoma Cell and Xenograft in Nude Mice[J];Chinese Journal of Cancer;2002-04
9 ZHANG Lin jie, FANG Yan, MA Ying hong, YAN Jan, ZHANG Xiao shi, HUANG Bi jun, ZENG Yi xin*Department of Etiology,Cancer Institute,Cancer Center,Sun Yat sen University,Guangzhou 510060,P.R.China;Gene Expression Profiling in Nasopharyngeal Carcinoma Determined by High Density cDNA Microarray[J];Chinese Journal of Cancer;2002-06
10 Yang Hai Feng, Tang Wei PingDepartment of Pathology,GuangdongMedical College,Zhanjiang,Guangdong,524023,P.R.China;Vascular Endothelial Growth Factor Gene Expression in Nasopharyngeal Carcinoma Cell Line Induced by Hypoxia In Vitro[J];Chinese Journal of Cancer;2003-02
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 REN Yan-fang,LIU Chang,XU Ling-ling,HE Jun-yu(College of Agriculture,Guizhou University,Guiyang,Guizhou 550025);Studies on Inhibitory Effects of Herbal Extracts Against Postharvest Pathogen from Citrus Fruits[J];Northern Horticulture;2010-23
2 Chai Hui,Yu Junwei,Ma Jianlong,et al Life Science Faculty of Zhejiang University of Traditional Chinese Medicine,Hangzhou(310053),China;Effect of Berberine in Tumor Necrosis Factor-α Induced Inflammation[J];Journal of Zhejiang Chinese Medical University;2011-04
3 Zhang Li;Therapeutic effect of hydropathic compress of mahuang tincture on paronychia[J];Journal of Nursing Science;2010-12
4 DING Yi-wei,Check: GUO Jian-wei,MA Cong Navy Total Hospital,Beijing,China,100048;Investigation on New Antibacterials From Effective Compontents of Chinese Herbs[J];Guiding Journal of Traditional Chinese Medicine and Pharmacy;2009-06
5 ;Research process on pharmacological action of berberine[J];Journal of Jilin Medical College;2008-05
6 Long Wei,Ma Shitang,Liu Peixun~*,Yu Jie,Xu Yang,Hong Ge and Li Xinru (Institute of Radiation Medicine,Chinese Academy of Medicine Science,Peking Union Medical College,Tianjin,300192, China);The multi-target research on the material basis of anti-inflammation effect in HLJD[J];Computers and Applied Chemistry;2009-07
7 YU Xiao-dong,WANG Zhi-hui,ZHAO Hong-xin,CHEN Li-xin,WU Lian-jie,ZHAO Shi-cheng (Tangshan Fengrun District Hospital of TCM,Tangshan 064000,Heibei,China);Clincial Observation on GuiXiang Paste for Treatment of Limbs End Mixed Gangrene of Diabetes[J];Journal of Liaoning University of Traditional Chinese Medicine;2010-08
8 ZHAO Yun-long,FENG Bei,ZHOU Yi,CAO Shi-xia(Institute of Basic Medical Sciences of Chengdu TCM University,Chengdu 611130,Sichuan,China);The Protective Effects of Coptis Chinensis Franch and C.Deltoidea C.Y. Chenget Hsiao on Endotoxin Challenged Mice[J];Liaoning Journal of Traditional Chinese Medicine;2011-07
9 ZHANG Xu, QIAO Ji-ying, ZHANG Rong, WEI Zhi-chao, CAI Juan (Department of Immunology and Pathogenic Biology, Medical School of Xi'an Jiaotong University, Xi'an 710061, China);In Vitro Antiprotozoal Effects of Brucea javanica,Coptis chinensis, Radix pulsatillae and Arecae on Blastocystis hominis[J];Journal of Tropical Medicine;2007-11
10 CHENG Kang, FANG Tai -hui, ZHAO-Ya, ZHU-Le, WEI Shao-min (1. Nanjin University of Traditional Chinese Medicine, Nanjin 210029, China; 2. Shanghai Jahwa United Co. Ltd.,Shanghai 201702,China );Experiment study of "Qinyanruyu Decotion" on curing acne vulgaris[J];Detergent & Cosmetics;2007-06
China Proceedings of conference Full-text Database 2 Hits
1 CHENG Kang Zhao-ya Zhu-le WEI Shao-min (Shanghai Jahwa United Co.Ltd);Experiment study of ‘Qinyanruyu Decotion’on curing acne vulgaris[A];[C];2008
2 XIAO Xue-feng~1,GAO Lan~1,MO Fan~1,LIU Chang-xiao~(2*) 1 Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China;2 Tianjian State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research,Tianjin 300193,China;Study on pharmacokinetics of Berberine Hydrochloride in Huanglianjiedu Decoction[A];[C];2009
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