Expression of p27 in rat kidney with unilateral ureteral obstruction and the therapeutic effect of enalapril
SUN Jian~ 1 ,TAO Li-jian~ 1,* , JIN Ou~ 2 , NING Wang-bin~ 1 ,DAMU Tang~ 3 (1.Department of Nephrology, Xiangya Hospital, Central South University; 2.Department of Pathology, Basic Medical College, Central South University, Changsha 410008;3.Department of Medicine, McMaster University, Hamilton, Ontario L8N 4A6, Canada)
Objective To explore the effect of p27 in the renal tubule on the process of renal interstitial fibrosis caused by unilateral ureteral obstruction (UUO) in rats,and to examine the expression changes of p27 after enalapril intervention and to interpret the anti-fibrotic mechanism.Methods Ninety rats were randomly divided into the sham-operated group (SOR), UUO group, and UUO+enalapril treatment group enalapril: 10mg/(kg·d). The rats of each group were respectivedly sacrificed on 7, 14, 21 days post-operatively. The renal pathological changes were dynamically observed by HE . The expression and dynamic changes of p27 were detected by immunohistochemistry. The level of p27 mRNA were detected by RT-PCR. Results The expression of p27 in renal tubular epithelial cells and p27 mRNA were strongly positive in the SOR group. With degree of interstitial fibrosis aggravating, the expression of p27 mRNA was gradually reducing. Enalapril could improve the expression of p27 on the 14th and 21st days after the UUO. Conclusion (1)This study supports a causative role of p27 in the formation of fibrosis of renal mesenchyme in rats with UUO. (2) The anti-fibrotic mechanism of enalapril is partly the improvement of p27 expression.
【CateGory Index】： R692