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《Journal of Central South University(Medical Sciences)》 2007-06
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Differential proteomic analysis of naspharyngeal carcinoma cell lines 5-8F and 6-10B

XIANG Ya-li1,2,YI Hong1,LI Cui1,ZHANG Peng-fei1,LI Mao-yu1,CHEN Zhu-chu1,XIAO Zhi-qiang1(1.Key Laboratory of Cancer Proteomics of Ministry of Health of China,Xiangya Hospital,Central SouthUniversity,Changsha 410008;2.Department of Pathology,Shajing People's Hospital,Shenzhen 518104,China)  
Objective To compare the proteome difference of nasopharyngeal carcinoma(NPC)cell lines 5-8F and 6-10B,and to screen these proteins associated with NPC metastasis.Methods Two-dimensional gel electrophoresis(2-DE)was used to separate the total proteins from NPC cell lines 5-8F and 6-10B with different metastatic potentials and same genetic background,respectively.PDQuest software was applied to analyze 2-DE images,and the differentially expressed protein spots between 5-8F and 6-10B were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS).The expression levels of partial identified proteins in the 2 cell lines were detected by Western blot.Results 2-DE maps of total proteins from 5-8F and 6-10B were established.A total of 65 differential protein spots in the 2 cell lines were found,and 15 non-redundant differential expression proteins were identified by MALDI-TOF-MS.Western blot showed that Annexin A1 and 14-3-3 protein sigma were differential expression proteins in 5-8F and 6-10B,which was consistent with the results from the comparative proteomic analysis.Conclusion Fifteen non-redundant differential expression proteins are useful for studying the metastatic mechanism of NPC.
【Fund】: 国家重点基础研究发展计划(973)资助项目(2001CB510207);; 湖南省科技厅重点科研项目(06SK2004)~~
【CateGory Index】: R739.6
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