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Effect of different concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats

ZHAO Shuangping,WU Jiao,GUO Qulian,ZHANG Zhong,YE Zhi(Intensive Care Unit,Xiangya Hospital,Central South University,Changsha 410008,China)  
Objective To investigate the effect of 3 concentrations of sevoflurane pretreatment on acute lung injury induced by endotoxin in rats.Methods Thirty-six male SD rats were randomly divided into 6 groups:a control group(Group A),a sevoflurane group(Group B),a 3.6% sevoflurane pretreatment group(Group C),a 2.4% Sevoflurane pretreatment group(Group D),a 1.2% sevo sevoflurane pretreatment group(Group E),and lipopolysaccharide(Group F).The rats were killed 6 h after lipopolysaccharide(LPS) or saline injection.Histological examinations of the lung tissues were performed with light microscope.Lung wet/dry weight(W/D) ratio,myeloperoxidase(MPO) activity,and intercellular adhesion molecule-1(ICAM-1) mRNA expression were assayed.Results Introvenous LPS significantly aggravated lung tissue damage,increased lung W/D ratio,MPO activity,and lung ICAM-1 mRNA expression compared with the control group(P0.05).Precondition with 2.4% sevoflurane significantly attenuated the above-mentioned changes induced by LPS(P0.05).The 3.6% LPS(Group C) significantly attenuated lung tissue damage and decreased MPO activity,lung ICAM-1 mRNA expression compared with the Group F(P0.05),but no significant change in lung W/D ratio was seen(P0.05).MPO activity was significantly decreased in Group E(P0.05),but lung W/D ratio and lung ICAM-1 mRNA expression had no significant changes(P0.05).Conclusion Precondition with 2.4% sevoflurane can reduce LPS-induced lung injury in rats.Decreased expression of ICAM-1 and less accumulation of neutrophils were participated in its mechanism.
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